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Information Journal Paper

Title

EVALUATION OF HUMAN BREAST ADENOCARCINOMA (MCF-7) CELLS PROLIFERATION IN CO-CULTURE WITH HUMAN ADIPOCYTES IN THREE DIMENSIONAL COLLAGEN GEL MATRIX: NOREPINEPHRINE AS A LIPOLYTIC FACTOR

Pages

  125-131

Keywords

HUMAN BREAST ADENOCARCINOMA (MCF-7) CELL 

Abstract

 Background: NOREPINEPHRINE plays a trophic role in the control of cell replication and differentiation in target cells that express adrenergic receptors. Methods: In this study, we have tested the influence of infraphysiological, physiological and supraphysiological concentrations (0.0001 nM, 1 nM, 10000 nM) of human NOREPINEPHRINE on the PROLIFERATION of breast cancer cells (human breast adenocarcinoma [MCF-7]) in co-culture with HUMAN ADIPOCYTEs in three-dimensional collagen gel matrix culture. Cell PROLIFERATION and LIPOLYSIS rate were measured by 3-(4, 5-dimethylthiazolyl)-2, 5-diphenyl-tetrazolium bromide (MTT) and Oil red O colorimetric assay in second, 7th and 14th days of culture experiments.Results: Our results showed a direct correlation between LIPOLYSIS rate of adipocytes and PROLIFERATION rate of MCF-7 cells. Both physiological and supraphysiological concentrations of human NOREPINEPHRINE significantly (P<0.05) increased the PROLIFERATION of MCF-7 cells synchronously with progress of adipocyte LIPOLYSIS. The PROLIFERATIONs of MCF-7 cells were significantly decreased after conversion of adipocytes to fibroblast-like cells by supraphysiological concentration of NOREPINEPHRINE. There was no statistical difference in LIPOLYSIS of adipocytes and PROLIFERATION of MCF-7 cells in response to infraphysiological concentration of NOREPINEPHRINE. Conclusion: These findings indicated that NOREPINEPHRINE stimulated the PROLIFERATION of MCF-7 cells in coculture with HUMAN ADIPOCYTEs as a lipolytic factor and that NOREPINEPHRINE effect was suppressed by conversion of adipocytes to fibroblast-like cells, suggesting adipocytes as another target for prevention and therapy of breast cancer.  

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    APA: Copy

    BARBARESTANI, M., RAGERDI KASHANI, I., ETESAM, F., ABD ALVAHABI, M.A., NOURI MOKOHI, M.H., SHOKRGOZAR, M.A., HADAD, P., & HOSSEINI, M.. (2006). EVALUATION OF HUMAN BREAST ADENOCARCINOMA (MCF-7) CELLS PROLIFERATION IN CO-CULTURE WITH HUMAN ADIPOCYTES IN THREE DIMENSIONAL COLLAGEN GEL MATRIX: NOREPINEPHRINE AS A LIPOLYTIC FACTOR. IRANIAN BIOMEDICAL JOURNAL, 10(3), 125-131. SID. https://sid.ir/paper/277114/en

    Vancouver: Copy

    BARBARESTANI M., RAGERDI KASHANI I., ETESAM F., ABD ALVAHABI M.A., NOURI MOKOHI M.H., SHOKRGOZAR M.A., HADAD P., HOSSEINI M.. EVALUATION OF HUMAN BREAST ADENOCARCINOMA (MCF-7) CELLS PROLIFERATION IN CO-CULTURE WITH HUMAN ADIPOCYTES IN THREE DIMENSIONAL COLLAGEN GEL MATRIX: NOREPINEPHRINE AS A LIPOLYTIC FACTOR. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2006;10(3):125-131. Available from: https://sid.ir/paper/277114/en

    IEEE: Copy

    M. BARBARESTANI, I. RAGERDI KASHANI, F. ETESAM, M.A. ABD ALVAHABI, M.H. NOURI MOKOHI, M.A. SHOKRGOZAR, P. HADAD, and M. HOSSEINI, “EVALUATION OF HUMAN BREAST ADENOCARCINOMA (MCF-7) CELLS PROLIFERATION IN CO-CULTURE WITH HUMAN ADIPOCYTES IN THREE DIMENSIONAL COLLAGEN GEL MATRIX: NOREPINEPHRINE AS A LIPOLYTIC FACTOR,” IRANIAN BIOMEDICAL JOURNAL, vol. 10, no. 3, pp. 125–131, 2006, [Online]. Available: https://sid.ir/paper/277114/en

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