IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS CTL EPITOPES RESTRICTED BY HLA-A*0201 IN HHD MICE

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RAFIEI ALI REZA; KIUTADE Y.

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Journal: Iranian Biomedical Journal Year:2007 | Volume:11 | Issue:1 Page(s): 23-31

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Title

IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS CTL EPITOPES RESTRICTED BY HLA-A*0201 IN HHD MICE

Author(s)

RAFIEI ALI REZA | KIUTADE Y. | Issue Writer Certificate

Keywords

DNA VACCINE

MPB51

HHD MICE

EPITOPE

Abstract

Background: CD8+ T cells are thought to play an important role in protective immunity to tuberculosis. The major histocompatibility complex class I subtype HLA-A*0201 is one of the most prevalent class I alleles, with a frequency of over 30% in most populations. HLA-A*0201 transgenic, H-2Db/mouse beta2- microglobulin double-knockout mice (HHD) which express human HLA-A*0201 but no mouse class I, was shown to provide a powerful model for studying induction of HLA-A*0201-restricted immune responses in vivo. Methods: HHD MICE were immunized with plasmid DNA encoding MPB51 by using a gene gun, and IFN-g production from the immune spleen cells was analyzed in response to a synthetic overlapping peptide library covering the mature MPB51 sequence. catatonic T lymphocytes (CTL) activity was measured using cytotoxicity assay and the three-color flowcytometry was used to reveal IFN-g-producing immune spleen cells. Results: Our findings were shown that only one peptide, p51-70, appeared to stimulate the immune splenocytes to produce IFN-g. Flow cytometric analysis with intracellular IFN-g and the T-cell phenotype revealed that the p51-70 peptide contains an immunodominant CD8+ T-cell EPITOPE. Further analysis with computer-assisted algorithms permitted identification of a T-cell nona mer EPITOPE, p54-62. Finally, we proved that the p54-62/HLA-A*0201 complex is strongly recognized by HLA class I-restricted CD8+ MPB51-specific CTL cells. Conclusion: These results suggest that vaccination with MPB51 gene elicited MPB51-specific CTL. In addition, the P54-62 EPITOPE thus represent potential subunit component for the design of vaccines against tuberculosis.

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APA: Copy

RAFIEI, ALI REZA, & KIUTADE, Y.. (2007). IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS CTL EPITOPES RESTRICTED BY HLA-A*0201 IN HHD MICE. IRANIAN BIOMEDICAL JOURNAL, 11(1), 23-31. SID. https://sid.ir/paper/277128/en

Vancouver: Copy

RAFIEI ALI REZA, KIUTADE Y.. IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS CTL EPITOPES RESTRICTED BY HLA-A*0201 IN HHD MICE. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2007;11(1):23-31. Available from: https://sid.ir/paper/277128/en

IEEE: Copy

ALI REZA RAFIEI, and Y. KIUTADE, “IDENTIFICATION OF MYCOBACTERIUM TUBERCULOSIS CTL EPITOPES RESTRICTED BY HLA-A*0201 IN HHD MICE,” IRANIAN BIOMEDICAL JOURNAL, vol. 11, no. 1, pp. 23–31, 2007, [Online]. Available: https://sid.ir/paper/277128/en

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