THE PROTECTIVE EFFECTS OF N-ACETL-CYSTEINE, OXO-THIAZOLIDINE- CARBOXYLATE, ACETAMINOPHEN AND THEIR COMBINATIONS AGAINST SULFUR MUSTARD CYTOTOXICITY ON HUMAN SKIN FIBROBLAST CELL LINE (HF2FF)

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SABERI MEHDI; ZAREE MAHMODABADY ALI

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2009 | Volume:13 | Issue:4 Page(s): 215-221

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Title

THE PROTECTIVE EFFECTS OF N-ACETL-CYSTEINE, OXO-THIAZOLIDINE- CARBOXYLATE, ACETAMINOPHEN AND THEIR COMBINATIONS AGAINST SULFUR MUSTARD CYTOTOXICITY ON HUMAN SKIN FIBROBLAST CELL LINE (HF2FF)

Author(s)

SABERI MEHDI | ZAREE MAHMODABADY ALI | Issue Writer Certificate

Keywords

SULFUR MUSTARD (SM)

SKIN FIBROBLAST CELLS

N-ACETYL-CYSTEINE

2-OXO-THIAZOLIDINE-4-CARBOXYLATE (OTC)

ACETAMINOPHEN

Abstract

Background: Using human skin-fibroblast cell line HF2FF, the efficacy of some drugs was evaluated against sulfur mustard (SM) cytotoxicity. The drugs were the sulfhydryl containing molecule including Nacetylcysteine (NAC), 2-oxo-thiazolidine-4-carboxylate (OTC) and ACETAMINOPHEN as glutathione (GSH) stimulator pathway.Methods: The protective effects of NAC (0.1 mM), OTC (1.8 mM), and ACETAMINOPHEN (25 mM) alone or in combination with each other were evaluated on SM (180mM) -induced cytotoxicity. NAC and OTC were applied with SM simultaneously and ACETAMINOPHEN 30 min before SM exposure, incubated for 1 h and then were rinsed and incubated with fresh medium. The efficacy was evaluated by determination of cells viability, intracellular GSH level and catalase activity 1 and 24 h post SM exposure or co-treatments.Results: The cells viability was decreased 21.8% and 55.2%, respectively for 1 and 24 h post SM (1 h exposure) incubation. So, the 1-h SM exposure and 24-h treatment incubation were selected for evaluation.While, NAC alone treatment increased the cells viability (25%), GSH level (320%) and catalase activity (18%), the most effective combination was NAC plus OTC and ACETAMINOPHEN which increased more significantly the cells viability (about 40%), GSH level (470%) and catalase activity (100%).Conclusion: The most effective combination was NAC (0.1 mM) plus OTC (1.8 mM) and ACETAMINOPHEN (25 mM) which should be used before or concomitant with SM exposure. These drugs may reduce SM toxicity possibly by increment of GSH level and catalase activity. This efficacy needs to be confirmed byin vivo study.

Cites

COMPARISON OF PROTECTIVE EFFECTS OF N-ACETYL-CYSTEINE AND HEXAMETHYLENETETRAMINE ON SULFUR MUSTARD INDUCED PATHOLOGICAL EFFECTS IN HUMAN SKIN FIBROBLAST CELL LINE HF2FF USING ELECTRON MICROSCOPE

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APA: Copy

SABERI, MEHDI, & ZAREE MAHMODABADY, ALI. (2009). THE PROTECTIVE EFFECTS OF N-ACETL-CYSTEINE, OXO-THIAZOLIDINE- CARBOXYLATE, ACETAMINOPHEN AND THEIR COMBINATIONS AGAINST SULFUR MUSTARD CYTOTOXICITY ON HUMAN SKIN FIBROBLAST CELL LINE (HF2FF). IRANIAN BIOMEDICAL JOURNAL, 13(4), 215-221. SID. https://sid.ir/paper/277246/en

Vancouver: Copy

SABERI MEHDI, ZAREE MAHMODABADY ALI. THE PROTECTIVE EFFECTS OF N-ACETL-CYSTEINE, OXO-THIAZOLIDINE- CARBOXYLATE, ACETAMINOPHEN AND THEIR COMBINATIONS AGAINST SULFUR MUSTARD CYTOTOXICITY ON HUMAN SKIN FIBROBLAST CELL LINE (HF2FF). IRANIAN BIOMEDICAL JOURNAL[Internet]. 2009;13(4):215-221. Available from: https://sid.ir/paper/277246/en

IEEE: Copy

MEHDI SABERI, and ALI ZAREE MAHMODABADY, “THE PROTECTIVE EFFECTS OF N-ACETL-CYSTEINE, OXO-THIAZOLIDINE- CARBOXYLATE, ACETAMINOPHEN AND THEIR COMBINATIONS AGAINST SULFUR MUSTARD CYTOTOXICITY ON HUMAN SKIN FIBROBLAST CELL LINE (HF2FF),” IRANIAN BIOMEDICAL JOURNAL, vol. 13, no. 4, pp. 215–221, 2009, [Online]. Available: https://sid.ir/paper/277246/en

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