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Information Journal Paper

Title

THE EFFECTS OF FIBROBLAST CO-CULTURE AND ACTIVIN A ON IN VITRO GROWTH OF MOUSE PREANTRAL FOLLICLES

Pages

  49-53

Abstract

 Background: This study was conducted to evaluate FIBROBLAST co-culture and ACTIVIN A on in vitro maturation and fertilization of mouse preantral FOLLICLEs.Methods: The ovaries from 12-14-day-old mice were dissected, and 120-150 mm preantral FOLLICLEs were cultured individually in a-MEM as based medium for 12 days. A total number of 456 FOLLICLEs were cultured in four conditions: (i) base medium as control group (n=113), (ii) base medium supplemented with 30 ng/ml ACTIVIN A (n=115), (iii) base medium co-cultured with mouse embryonic FIBROBLAST (n=113), and (iv) base medium supplemented with 30 ng/ml ACTIVIN A and co-cultured with FIBROBLAST (n=115). Rate of growth, survivability, antrum formation, ovulation, embryonic development and steroid production were evaluated. Analysis of Variance and Duncan test were applied for analyzing.Results: Both co-culture and coculture+Activin A groups showed significant difference (P<0.05) in growth (on days 4, 6, and 8 of culture period) and survival rates. However, there was no significant difference in antrum formation, ovulation rate, and embryonic development of ovulated oocytes. There were significant differences (P<0.05) in the estradiol production on days 8, 10, and 12 between co-culture+Activin A and the control group. Progesterone production also was significant (P<0.05) in co-culture+Activin A group on days 6, 8, 10, and 12 compared to control group.Conclusion: FIBROBLAST co-culture and ACTIVIN A promoted growth and survivability of preantral FOLLICLEs. However, simultaneous use of them was more efficient.

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    • Cite

    APA: Copy

    KARIMPOUR MALEKSHAH, ABBASALI, HEIDARI, MAHMOUD, PARIVAR, KAZEM, & AZAMI, NASRIN SADAT. (2014). THE EFFECTS OF FIBROBLAST CO-CULTURE AND ACTIVIN A ON IN VITRO GROWTH OF MOUSE PREANTRAL FOLLICLES. IRANIAN BIOMEDICAL JOURNAL, 18(1), 49-53. SID. https://sid.ir/paper/277322/en

    Vancouver: Copy

    KARIMPOUR MALEKSHAH ABBASALI, HEIDARI MAHMOUD, PARIVAR KAZEM, AZAMI NASRIN SADAT. THE EFFECTS OF FIBROBLAST CO-CULTURE AND ACTIVIN A ON IN VITRO GROWTH OF MOUSE PREANTRAL FOLLICLES. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2014;18(1):49-53. Available from: https://sid.ir/paper/277322/en

    IEEE: Copy

    ABBASALI KARIMPOUR MALEKSHAH, MAHMOUD HEIDARI, KAZEM PARIVAR, and NASRIN SADAT AZAMI, “THE EFFECTS OF FIBROBLAST CO-CULTURE AND ACTIVIN A ON IN VITRO GROWTH OF MOUSE PREANTRAL FOLLICLES,” IRANIAN BIOMEDICAL JOURNAL, vol. 18, no. 1, pp. 49–53, 2014, [Online]. Available: https://sid.ir/paper/277322/en

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