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Information Journal Paper

Title

IMMUNOGENICITY OF A FUSION PROTEIN COMPRISING COLI SURFACE ANTIGEN 3 AND LABILE B SUBUNIT OF ENTEROTOXIGENIC ESCHERICHIA COLI

Pages

  212-218

Keywords

ENTER OTOXIGENIC ESCHERICHIA COLI (ETEC) 

Abstract

 Background: Enter otoxigenic Escherichia coli (ETEC) strains are the major causes of diarrheal disease in humans and animals. Colonization factors and enterotoxins are the major virulence factors in ETEC pathogenesis. For the broad-spectrum protection against ETEC, one could focus on colonization factors and non-toxic heat labile as a vaccine candidate. Methods: A fusion protein is composed of a major fimbrial subunit of coli surface antigen 3, and the heat-labile B subunit (LTB) was constructed as a chimeric immunogen. For optimum level expression of protein, the gene was synthesized with codon bias of E. coli. Also, recombinant protein was expressed in E. coli BL21DE3. ELISA and Western tests were carried out for determination of antigen and specificity of antibody raised against recombinant protein in animals. The anti-toxicity and anti-adherence properties of the immune sera against ETEC were also evaluated. Results: Immunological analyses showed the production of high titer of specific antibody in immunized mice. The built-in LTB retains native toxin properties which were approved by GM1 binding assay. Pre-treatment of the ETEC cells with anti-sera significantly decreased their adhesion to Caco-2 cells. Conclusion: The results indicated the efficacy of the recombinant chimeric protein as an effective immunogen inducing strong humoral response. The designated chimer would be an interesting prototype for a vaccine and worthy of further investigation.

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    APA: Copy

    ALERASOL, MASOOME, MOUSAVI GARGARI, SEYED LATIF, NAZARIAN, SHAHRAM, & BAGHERI, SAMANEH. (2014). IMMUNOGENICITY OF A FUSION PROTEIN COMPRISING COLI SURFACE ANTIGEN 3 AND LABILE B SUBUNIT OF ENTEROTOXIGENIC ESCHERICHIA COLI. IRANIAN BIOMEDICAL JOURNAL, 18(4), 212-218. SID. https://sid.ir/paper/277354/en

    Vancouver: Copy

    ALERASOL MASOOME, MOUSAVI GARGARI SEYED LATIF, NAZARIAN SHAHRAM, BAGHERI SAMANEH. IMMUNOGENICITY OF A FUSION PROTEIN COMPRISING COLI SURFACE ANTIGEN 3 AND LABILE B SUBUNIT OF ENTEROTOXIGENIC ESCHERICHIA COLI. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2014;18(4):212-218. Available from: https://sid.ir/paper/277354/en

    IEEE: Copy

    MASOOME ALERASOL, SEYED LATIF MOUSAVI GARGARI, SHAHRAM NAZARIAN, and SAMANEH BAGHERI, “IMMUNOGENICITY OF A FUSION PROTEIN COMPRISING COLI SURFACE ANTIGEN 3 AND LABILE B SUBUNIT OF ENTEROTOXIGENIC ESCHERICHIA COLI,” IRANIAN BIOMEDICAL JOURNAL, vol. 18, no. 4, pp. 212–218, 2014, [Online]. Available: https://sid.ir/paper/277354/en

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