EXPRESSION OF TWO BASIC MRNA BIOMARKERS IN PERIPHERAL BLOOD OF PATIENTS WITH NON-SMALL CELL LUNG CANCER DETECTED BY REAL-TIME RT-PCR, INDIVIDUALLY AND SIMULTANEOUSLY

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

KARIMI SHIRIN; MOHAMADNIA ABDOLREZA; NADJI SEYED ALIREZA; YADEGARAZARI REZA; KHOSRAVI ADNAN; BAHRAMI NAGHMEH; SAIDIJAM MASSOUD

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Iranian Biomedical Journal Year:2015 | Volume:19 | Issue:1 Page(s): 17-22

Download Full-Text

View:

400

Download:

214

Cites:

Information Journal Paper

Title

EXPRESSION OF TWO BASIC MRNA BIOMARKERS IN PERIPHERAL BLOOD OF PATIENTS WITH NON-SMALL CELL LUNG CANCER DETECTED BY REAL-TIME RT-PCR, INDIVIDUALLY AND SIMULTANEOUSLY

Author(s)

Keywords

LUNG NEOPLASMS

RNA

CARCINOEMBRYONIC ANTIGEN

SENSITIVITY AND SPECIFICITY

Abstract

Introduction: Although extensive research has been conducted on lung cancer markers, a singular clinically applicable marker has not been found yet. The objective of this study was to evaluate the sensitivity and the specificity of CARCINOEMBRYONIC ANTIGEN (CEA) mRNA and lung-specific X protein (LUNX) mRNA biomarkers in peripheral blood to detect lung cancer individually and simultaneously.Methods: Thirty patients affected by lung cancer and 30 healthy individuals were studied in this research. Three vials of cDNA were made from each sample after taking peripheral blood samples and extracting total RNA. Each sample was examined by the real-time RTPCR technique. The result from each vial was then compared with the sensitivity of overall marker.Results: The CEA mRNA was positive in 24 out of 30 lung cancer patients. Hence, its sensitivity was determined at 80%, differing significantly from that observed in healthy individuals, where 11 positive cases were seen. The overall sensitivity of this marker was significantly associated with positivity in vials 2 and 3 but not in vial 1. The LUNX mRNA was positive in 21 out of 30 patients, indicating 70% sensitivity. This finding significantly differed from that in healthy individuals. The overall sensitivity of this marker was significantly associated with positivity in vials 1 and 3, but not in vial 2. In 93.3% of the patients, at least one positive marker was observed.Conclusion: The mentioned mRNA could be suggested as sensitive and specific markers in peripheral blood for primary diagnosis of lung cancer.

Cites

DETECTION OF VASCULAR ENDOTHELIAL GROWTH FACTOR MESSENGER RNA (VEGF MRNA), CYTOKERATIN-19 MRNA (CK19 MRNA), AND VASCULAR ENDOTHELIAL GROWTH FACTOR (VEGF) PROTEIN BIOMARKER IN THE PERIPHERAL BLOOD OF PATIENTS WITH BREAST CANCER

References

IMPROVED REAL-TIME RT-PCR ASSAYS OF TWO COLORECTAL CANCER PERIPHERAL BLOOD MRNA BIOMARKERS: A PILOT STUDY

Cite

APA: Copy

KARIMI, SHIRIN, MOHAMADNIA, ABDOLREZA, NADJI, SEYED ALIREZA, YADEGARAZARI, REZA, KHOSRAVI, ADNAN, BAHRAMI, NAGHMEH, & SAIDIJAM, MASSOUD. (2015). EXPRESSION OF TWO BASIC MRNA BIOMARKERS IN PERIPHERAL BLOOD OF PATIENTS WITH NON-SMALL CELL LUNG CANCER DETECTED BY REAL-TIME RT-PCR, INDIVIDUALLY AND SIMULTANEOUSLY. IRANIAN BIOMEDICAL JOURNAL, 19(1), 17-22. SID. https://sid.ir/paper/277385/en

Vancouver: Copy

KARIMI SHIRIN, MOHAMADNIA ABDOLREZA, NADJI SEYED ALIREZA, YADEGARAZARI REZA, KHOSRAVI ADNAN, BAHRAMI NAGHMEH, SAIDIJAM MASSOUD. EXPRESSION OF TWO BASIC MRNA BIOMARKERS IN PERIPHERAL BLOOD OF PATIENTS WITH NON-SMALL CELL LUNG CANCER DETECTED BY REAL-TIME RT-PCR, INDIVIDUALLY AND SIMULTANEOUSLY. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(1):17-22. Available from: https://sid.ir/paper/277385/en

IEEE: Copy

SHIRIN KARIMI, ABDOLREZA MOHAMADNIA, SEYED ALIREZA NADJI, REZA YADEGARAZARI, ADNAN KHOSRAVI, NAGHMEH BAHRAMI, and MASSOUD SAIDIJAM, “EXPRESSION OF TWO BASIC MRNA BIOMARKERS IN PERIPHERAL BLOOD OF PATIENTS WITH NON-SMALL CELL LUNG CANCER DETECTED BY REAL-TIME RT-PCR, INDIVIDUALLY AND SIMULTANEOUSLY,” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 1, pp. 17–22, 2015, [Online]. Available: https://sid.ir/paper/277385/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops