GLYCOCONJUGATES DISTRIBUTION DURING DEVELOPING MOUSE SPINAL CORD MOTOR ORGANIZERS (SHORT REPORT)

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VOJOUDI ELHAM; EBRAHIMI VAHID; EBRAHIMZADEH BIDESKAN ALIREZA; FAZEL ALIREZA

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Journal Paper

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Journal: Iranian Biomedical Journal Year:2015 | Volume:19 | Issue:1 Page(s): 63-68

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Information Journal Paper

Title

GLYCOCONJUGATES DISTRIBUTION DURING DEVELOPING MOUSE SPINAL CORD MOTOR ORGANIZERS (SHORT REPORT)

Author(s)

VOJOUDI ELHAM | EBRAHIMI VAHID | EBRAHIMZADEH BIDESKAN ALIREZA | FAZEL ALIREZA | Issue Writer Certificate

Keywords

DEVELOPMENT

GLYCOCONJUGATES

SPINAL CORD

Abstract

Background: The aim of this research was to study the distribution and changes of GLYCOCONJUGATES particularly their terminal sugars by using lectin histochemistry during mouse SPINAL CORD DEVELOPMENT.Methods: Formalin-fixed sections of mouse embryo (10-16 fetal days) were processed for lectin histochemical method. In this study, two groups of horseradish peroxidase- labeled specific lectins were used: N-acetylgalactosamine, including Dolichos biflorus, Wisteria floribunda agglutinin (WFA), Vicia villosa, Glycine max as well as focuse-binding lectins, including tetragonolobus, Ulex europaeus, and Orange peel fungus (OFA). All sections were counterstained with alcian blue (pH 2.5).Results: Our results showed that only WFA and OFA reacted strongly with the floor plate cells from early to late embryonic period of developing SPINAL CORD. The strongest reactions were related to the 14, 15, and 16 days of tissue sections incubated with OFA and WFA lectins.Conclusion: The present study demonstrated that cellular and molecular differentiation of the SPINAL CORD organizers is a wholly regulated process, anda-L-fucose, a-D-GalNAc, and a/b-D-GalNAc terminal sugars play a significant role during the prenatal SPINAL CORD DEVELOPMENT.

Cites

GLYCOCONJUGATES DISTRIBUTION IN THE LATERAL WALLS OF SPINAL CORD DURING MOUSE MORPHOGENESIS USING LECTIN HISTOCHEMICAL METHOD

References

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APA: Copy

VOJOUDI, ELHAM, EBRAHIMI, VAHID, EBRAHIMZADEH BIDESKAN, ALIREZA, & FAZEL, ALIREZA. (2015). GLYCOCONJUGATES DISTRIBUTION DURING DEVELOPING MOUSE SPINAL CORD MOTOR ORGANIZERS (SHORT REPORT). IRANIAN BIOMEDICAL JOURNAL, 19(1), 63-68. SID. https://sid.ir/paper/277392/en

Vancouver: Copy

VOJOUDI ELHAM, EBRAHIMI VAHID, EBRAHIMZADEH BIDESKAN ALIREZA, FAZEL ALIREZA. GLYCOCONJUGATES DISTRIBUTION DURING DEVELOPING MOUSE SPINAL CORD MOTOR ORGANIZERS (SHORT REPORT). IRANIAN BIOMEDICAL JOURNAL[Internet]. 2015;19(1):63-68. Available from: https://sid.ir/paper/277392/en

IEEE: Copy

ELHAM VOJOUDI, VAHID EBRAHIMI, ALIREZA EBRAHIMZADEH BIDESKAN, and ALIREZA FAZEL, “GLYCOCONJUGATES DISTRIBUTION DURING DEVELOPING MOUSE SPINAL CORD MOTOR ORGANIZERS (SHORT REPORT),” IRANIAN BIOMEDICAL JOURNAL, vol. 19, no. 1, pp. 63–68, 2015, [Online]. Available: https://sid.ir/paper/277392/en

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