A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM

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SHOKROLLAHI NARJES; SHAHBAZZADEH DELAVAR; POOSHANG BAGHERI KAMRAN; HABIBI ANBOUHI MAHDI; JAHANIAN NAJAFABADI ALI; BEHDANI MAHDI

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Journal: Iranian Biomedical Journal Year:2016 | Volume:20 | Issue:3 Page(s): 182-186

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Title

A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM

Author(s)

Keywords

BACULOVIRUS

SF9

TRANSFECTION

Abstract

Background: BACULOVIRUS expression system is one of the most attractive and powerful eukaryotic expression systems for the production of recombinant proteins. The presence of a biomarker is required to monitor TRANSFECTION efficiency or protein expression levels in insect cells.Methods: The aim of this study was to construct a BACULOVIRUS expression vector encoding a copepod super green fluorescent protein (copGFP). In this light, the resultant vector was constructed and used for TRANSFECTION of Spodoptera frugiperda cells.Results: Expression of the copGFP protein in insect cells was confirmed by fluorescent microscopy and Western-blot analysis.Conclusion: The application of copGFP control bacmid can be considered as an appropriate control for insect cell TRANSFECTION.

Cites

Cell-specific targeting by engineered M13 bacteriophage expressing VEGFR2 nanobody

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APA: Copy

SHOKROLLAHI, NARJES, SHAHBAZZADEH, DELAVAR, POOSHANG BAGHERI, KAMRAN, HABIBI ANBOUHI, MAHDI, JAHANIAN NAJAFABADI, ALI, & BEHDANI, MAHDI. (2016). A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM. IRANIAN BIOMEDICAL JOURNAL, 20(3), 182-186. SID. https://sid.ir/paper/277453/en

Vancouver: Copy

SHOKROLLAHI NARJES, SHAHBAZZADEH DELAVAR, POOSHANG BAGHERI KAMRAN, HABIBI ANBOUHI MAHDI, JAHANIAN NAJAFABADI ALI, BEHDANI MAHDI. A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM. IRANIAN BIOMEDICAL JOURNAL[Internet]. 2016;20(3):182-186. Available from: https://sid.ir/paper/277453/en

IEEE: Copy

NARJES SHOKROLLAHI, DELAVAR SHAHBAZZADEH, KAMRAN POOSHANG BAGHERI, MAHDI HABIBI ANBOUHI, ALI JAHANIAN NAJAFABADI, and MAHDI BEHDANI, “A MODEL TO STUDY THE PHENOTYPIC CHANGES OF INSECT CELL TRANSFECTION BY COPEPOD SUPER GREEN FLUORESCENT PROTEIN (COP-GFP) IN BACULOVIRUS EXPRESSION SYSTEM,” IRANIAN BIOMEDICAL JOURNAL, vol. 20, no. 3, pp. 182–186, 2016, [Online]. Available: https://sid.ir/paper/277453/en

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