مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

PRODUCTION OF A HUMAN RECOMBINANT ANTIBODY AGAINST SEROTYPE A CANDIDA ALBICANS

Pages

  273-278

Abstract

 After using 3 different generations of antibodies including human and non-human hyperimmune sera, monoclonal antibodies and chimeric antibodies, more recently a newer approach has been developed in which the antibody genes are cloned directly from a patient peripheral B-lymphocytes and expressed in a host like E. coli. In this study the CANDIDA ALBICANS serotype A (NCTC 3153) mannan was purified using a modified Fehling method and used for selection of HUMAN RECOMBINANT ANTIBODY from a C. albicans phage antibody library. After four rounds of affinity selecting (panning), 2 predominant clones were chosen by DNA fingerprinting and ELISA. A 248 amino acid DNA fragment coding for anti-C. albicans mannan SCFV was sequenced and cloned in a pBAD-TOPO cloning vector to produce a soluble and phage free antibody. The analysis of antibody sequences by V base Index (DNAPLOT) confirmed the human antibody origin with the VH4 family in V segment of heavy variable chain and VL3 (Lambda 3) in J segment of the light variable chain. This antibody fragment was purified using immobilized metal affinity chromatography and inmmunoblotted as a 31kDa recombinant protein.

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    Cite

    APA: Copy

    JAFARI, A.A.. (2005). PRODUCTION OF A HUMAN RECOMBINANT ANTIBODY AGAINST SEROTYPE A CANDIDA ALBICANS. ACTA MEDICA IRANICA, 43(4), 273-278. SID. https://sid.ir/paper/277861/en

    Vancouver: Copy

    JAFARI A.A.. PRODUCTION OF A HUMAN RECOMBINANT ANTIBODY AGAINST SEROTYPE A CANDIDA ALBICANS. ACTA MEDICA IRANICA[Internet]. 2005;43(4):273-278. Available from: https://sid.ir/paper/277861/en

    IEEE: Copy

    A.A. JAFARI, “PRODUCTION OF A HUMAN RECOMBINANT ANTIBODY AGAINST SEROTYPE A CANDIDA ALBICANS,” ACTA MEDICA IRANICA, vol. 43, no. 4, pp. 273–278, 2005, [Online]. Available: https://sid.ir/paper/277861/en

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