A COMPARATIVE STUDY OF THE SEPARATION OF OLEANOLIC ACID AND URSOLIC ACID IN PRUNELLA VULGARIS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

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DU H.; CHEN X.Q.

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Journal Paper

Paper Information

Journal: JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS) Year:2009 | Volume:6 | Issue:2 Page(s): 334-340

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Title

A COMPARATIVE STUDY OF THE SEPARATION OF OLEANOLIC ACID AND URSOLIC ACID IN PRUNELLA VULGARIS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY

Author(s)

DU H. | CHEN X.Q. | Issue Writer Certificate

Keywords

HPLC

CD-MEKC

PRUNELLA VULGARIS

OLEANOLIC ACID

URSOLIC ACID

Abstract

A high-performance liquid chromatographic (HPLC) method and a cyclodextrin-modified micellar electrokinetic chromatographic (CD-MEKC) method were developed to separate and determine OLEANOLIC ACID (OA) and URSOLIC ACID (UA) in PRUNELLA VULGARIS. HPLC separations were carried out on a Hedera ODS C18 column with methanol -H2O- acetic acid (85:15:0.3, v/v/v) as mobile phase at a flow-rate of 0.8 ml min-1. CD-MEKC analysis was performed on a CL1030 capillary electrophoresis system with a 6% (v/v) methanol solution (pH=9.0) containing 10 mM disodium tetraborate, 10 mM sodium dihydrogen phosphate, 50 mM sodium dodecylsulfate (SDS), 15 mM 2-hydroxypropyl-b-cyclodextrin (2-HP-b-CD) as background electrolyte. The analytical results of HPLC and CD-MEKC were compared with each other. CD-MEKC has better analytical efficiency for two components, and the analytical time (15 min) was shorter than that of HPLC (35 min).

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APA: Copy

DU, H., & CHEN, X.Q.. (2009). A COMPARATIVE STUDY OF THE SEPARATION OF OLEANOLIC ACID AND URSOLIC ACID IN PRUNELLA VULGARIS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), 6(2), 334-340. SID. https://sid.ir/paper/282301/en

Vancouver: Copy

DU H., CHEN X.Q.. A COMPARATIVE STUDY OF THE SEPARATION OF OLEANOLIC ACID AND URSOLIC ACID IN PRUNELLA VULGARIS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY. JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS)[Internet]. 2009;6(2):334-340. Available from: https://sid.ir/paper/282301/en

IEEE: Copy

H. DU, and X.Q. CHEN, “A COMPARATIVE STUDY OF THE SEPARATION OF OLEANOLIC ACID AND URSOLIC ACID IN PRUNELLA VULGARIS BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY AND CYCLODEXTRIN-MODIFIED MICELLAR ELECTROKINETIC CHROMATOGRAPHY,” JOURNAL OF THE IRANIAN CHEMICAL SOCIETY(JICS), vol. 6, no. 2, pp. 334–340, 2009, [Online]. Available: https://sid.ir/paper/282301/en

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