مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

2,801
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

0
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

EVALUATING THE EFFECTS OF NGF, RA AND SHH GROWTH FACTORS ON MURINE EMBRYONIC STEM CELL DIFFERENTIATION INTO NEURONS AND OLIGOCYTES

Pages

  349-357

Abstract

 Introduction: Embryonic stem (ES) cells are pluripotent cells that can differentiate into all types of specific adult cells and are used for cell therapy in certain diseases. It seems that the inductive factors may be used to produce NEURONs and gliocytes out of ES cells for the treatment of some nervous system diseases in future. In this research, pluripotent and undifferentiated MURINE embryonic cells were cultured and effects of the growth factors NGF, RA and SHH were studied for the induction of neural and glial DIFFERENTIATIONs.Materials & Methods: CCB ES cell line derived from  mouse strain 129 were cultured on inactivated embryonic fibroblasts from C57/BL6 mice and embryonic bodies (EBs) were pre-pared and transplanted onto culture plates covered by fibronectin and growth factors NGF, SHH and RA with respective concentrations of  50 and 100 ng/ml, 300 and 500 ng/ml and 1 μM and  in some groups bFGF with a concentration of 20 ng/ml were added to the specific culture medium for neural cell precursors to induce cell DIFFERENTIATION into neural cells. For studying DIFFERENTIATION into many types of NEURONs and oligodendrocyte, gene expression of specific neural genes such as nestin, Nkx2-2, Nurr1, S100 and Olig-2 were assessed by RT-PCR and immunocytochemistry assays were used to confirm the presence of MAP-2 protein.Results: In this study, genetic evaluations showed that each of the previously mentioned growth factors activate some molecular mechanisms that have essential effects on DIFFERENTIATION into different kinds of nervous system cells by affecting the expression of marker genes. Cytochemistry of these cells by monoclonal MAP-2 antibody showed the resultant cells have dendrites that are specific for neural cells or NEURONs.Conclusion: The findings indicated that pluripotent and undifferentiated CCB ES cells underwent DIFFERENTIATION into neural cells and oligodendrocytes under the effects of NGF, SHH and RA growth factors and this neural induction was confirmed by the presence of molecular and antigenic markers in these cells. Each of these factors was effective in the induction of DIFFERENTIATION of specialized neural cells and even different concentrations of the factors induced particular cell DIFFERENTIATIONs.

Cites

  • No record.
  • References

    Cite

    APA: Copy

    JAHANBAKHT, H., PARIVAR, KAZEM, AKHOUNDI, M.A., JEDI TEHRANI, MAHMOUD, MODARESSI, M.H., SADEGHI, M.R., KERAMATIPOUR, M., ZARNANI, A.H., & SALEHKHOU, SH.. (2007). EVALUATING THE EFFECTS OF NGF, RA AND SHH GROWTH FACTORS ON MURINE EMBRYONIC STEM CELL DIFFERENTIATION INTO NEURONS AND OLIGOCYTES. JOURNAL OF REPRODUCTION AND INFERTILITY, 7(4 (29)), 349-357. SID. https://sid.ir/paper/28292/en

    Vancouver: Copy

    JAHANBAKHT H., PARIVAR KAZEM, AKHOUNDI M.A., JEDI TEHRANI MAHMOUD, MODARESSI M.H., SADEGHI M.R., KERAMATIPOUR M., ZARNANI A.H., SALEHKHOU SH.. EVALUATING THE EFFECTS OF NGF, RA AND SHH GROWTH FACTORS ON MURINE EMBRYONIC STEM CELL DIFFERENTIATION INTO NEURONS AND OLIGOCYTES. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2007;7(4 (29)):349-357. Available from: https://sid.ir/paper/28292/en

    IEEE: Copy

    H. JAHANBAKHT, KAZEM PARIVAR, M.A. AKHOUNDI, MAHMOUD JEDI TEHRANI, M.H. MODARESSI, M.R. SADEGHI, M. KERAMATIPOUR, A.H. ZARNANI, and SH. SALEHKHOU, “EVALUATING THE EFFECTS OF NGF, RA AND SHH GROWTH FACTORS ON MURINE EMBRYONIC STEM CELL DIFFERENTIATION INTO NEURONS AND OLIGOCYTES,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 7, no. 4 (29), pp. 349–357, 2007, [Online]. Available: https://sid.ir/paper/28292/en

    Related Journal Papers

    Related Seminar Papers

  • No record.
  • Related Plans

  • No record.
  • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button