EFFECTS OF ARTIFICIAL EXTRACELLULAR MATRIX ON FUNCTION OF HUMAN ENDOMETRIAL EPITHELIAL CELLS IN VITRO

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GHAFARI M.

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Journal: Journal of Reproduction & Infertility Year:2000 | Volume:1 | Issue:2 (2) Page(s): 40-49

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Title

EFFECTS OF ARTIFICIAL EXTRACELLULAR MATRIX ON FUNCTION OF HUMAN ENDOMETRIAL EPITHELIAL CELLS IN VITRO

Author(s)

GHAFARI M. | Issue Writer Certificate

Keywords

HUMANQ4

ENDOMETRIUMQ3

ECMQ4

IN VITROQ4

Abstract

Introduction: The importance of extra cellular matrix (ECM) in development and function of different cells has been reported but little is known about its role in HUMAN endometrial epithelial cells. We have examined effects of artificial ECM (Matrigel) on the function of HUMAN endometrial epithelial cells using molecular techniques.Materials and methods: Endometrial samples were removed, with informed patient consent and Ethics Committee approval, from 17 previously fertile women undergoing total abdominal hysterectomy. The tissue was dissociated and centrifuged to provide an epithelial rich suspension which was cultured either on plastic or seeded into Matrigel to produce polarized cells and then supplemented with or without progesterone (10-6 M). The amount of nucleic acid content of the cells in both IN VITRO model systems was examined by DNA, RNA extraction methods. The DNA and RNA content were later measured by spectrophotometer.Results: The amount of total RNA in cells grown on Matrigel (23±1.5 pg/cell) was more than double that in cells grown on plastic (9.1±1.4 pg/cell). Cells cultured on both IN VITRO model systems had RNA induced by steroid hormones, but the extent of induction was greater in cells grown on ECM than those on plastic. Cells cultured on ECM were differentiated and became polarized but cells grown on plastic proliferated until full Confluency.Conclusion: These results suggest that ECM plays an important role in gene expression, polarization and differentiation of HUMAN endometrial epithelial cells IN VITRO.

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APA: Copy

GHAFARI, M.. (2000). EFFECTS OF ARTIFICIAL EXTRACELLULAR MATRIX ON FUNCTION OF HUMAN ENDOMETRIAL EPITHELIAL CELLS IN VITRO. JOURNAL OF REPRODUCTION AND INFERTILITY, 1(2 (2)), 40-49. SID. https://sid.ir/paper/28555/en

Vancouver: Copy

GHAFARI M.. EFFECTS OF ARTIFICIAL EXTRACELLULAR MATRIX ON FUNCTION OF HUMAN ENDOMETRIAL EPITHELIAL CELLS IN VITRO. JOURNAL OF REPRODUCTION AND INFERTILITY[Internet]. 2000;1(2 (2)):40-49. Available from: https://sid.ir/paper/28555/en

IEEE: Copy

M. GHAFARI, “EFFECTS OF ARTIFICIAL EXTRACELLULAR MATRIX ON FUNCTION OF HUMAN ENDOMETRIAL EPITHELIAL CELLS IN VITRO,” JOURNAL OF REPRODUCTION AND INFERTILITY, vol. 1, no. 2 (2), pp. 40–49, 2000, [Online]. Available: https://sid.ir/paper/28555/en

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