DEVELOPMENT AND USE A NOVEL COMBINED IN-VIVO AND IN-VITRO ASSAY FOR ANTI-INFLAMMATORY AND IMMUNOSUPPRESSIVE AGENTS

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TAN LI; CHEN HONG; MEI XIN; WEI NA; CAO BO

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Journal: Iranian Journal of Pharmaceutical Research Year:2013 | Volume:12 | Issue:3 Page(s): 445-455

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Title

DEVELOPMENT AND USE A NOVEL COMBINED IN-VIVO AND IN-VITRO ASSAY FOR ANTI-INFLAMMATORY AND IMMUNOSUPPRESSIVE AGENTS

Author(s)

Keywords

IMMUNOREGULATION

DELAYED HYPERSENSITIVITY

T LYMPHOCYTES

IN-VITRO

Abstract

Contact hypersensitivity (CHS) mouse model induced by 2, 4-dinitrofluorobenzene (DNFB) is thought to be a T helper 1 (Th1)-dominant response and used for investigating anti-inflammatory and immunosuppressive agents. However, it is hardly used for screening large-scale drugs because of the large number of animals and complex mechanisms involved in-vivo. In this study, we focused on whether T LYMPHOCYTES from CHS mouse model could maintain the state of immune response IN-VITRO and explored a suitable time for drugs screening. The results showed that CD4+ T cells of CHS mice were higher compared with those in normal group. The expression of T-bet and GATA3 showed a Th1 shift and the levels of interleukin (IL)-2 and IL-4 also showed similar trend. Furthermore, IL-6 produced by T LYMPHOCYTES from CHS mice had a high level too. Then, we detected the effects of dexamethasone (DEX), cyclosporine A (CsA) and mycophenolate mofetil (MMF) on T LYMPHOCYTES IN-VITRO, and the data displayed that these immunosuppressive drugs could all inhibit the proliferation of T LYMPHOCYTES significantly. These findings suggested that T LYMPHOCYTES from CHS mice could mimic a similar immune response IN-VITRO, and it’s also a suitable method for screening anti-inflammatory and immunosuppressive agents.

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APA: Copy

TAN, LI, CHEN, HONG, MEI, XIN, WEI, NA, & CAO, BO. (2013). DEVELOPMENT AND USE A NOVEL COMBINED IN-VIVO AND IN-VITRO ASSAY FOR ANTI-INFLAMMATORY AND IMMUNOSUPPRESSIVE AGENTS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(3), 445-455. SID. https://sid.ir/paper/288109/en

Vancouver: Copy

TAN LI, CHEN HONG, MEI XIN, WEI NA, CAO BO. DEVELOPMENT AND USE A NOVEL COMBINED IN-VIVO AND IN-VITRO ASSAY FOR ANTI-INFLAMMATORY AND IMMUNOSUPPRESSIVE AGENTS. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(3):445-455. Available from: https://sid.ir/paper/288109/en

IEEE: Copy

LI TAN, HONG CHEN, XIN MEI, NA WEI, and BO CAO, “DEVELOPMENT AND USE A NOVEL COMBINED IN-VIVO AND IN-VITRO ASSAY FOR ANTI-INFLAMMATORY AND IMMUNOSUPPRESSIVE AGENTS,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. 3, pp. 445–455, 2013, [Online]. Available: https://sid.ir/paper/288109/en

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