مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

319
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

119
مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

PRODUCTION AND QUALITY CONTROL OF [67GA]-DOTA-TRASTUZUMAB FOR RADIOIMMUNOSCINTIGRAPHY

Pages

  355-366

Abstract

 Breast cancer radioimmunoscintigraphy targeting HER2/neu expression is a growing field of work in nuclear medicine research. In this study, TRASTUZUMAB was successively labeled with [67Ga] GaCl3 after CONJUGATION with DOTA-NHS-ester.The conjugates were purified by molecular filtration, the average number of DOTA conjugated per mAb was calculated and total concentration was determined by spectrophotometric method. DOTA-Trastuzumab was labeled with 67Ga. Radiochemical purity, integrity of protein after RADIOLABELING and stability of 67Ga-DOTA-Trastuzumab were determined followed by BIODISTRIBUTION studies in wild-type rats (30±5.5 mCi, 2, 4 and 24 h p.i.).The radioimmunoconjugate was prepared with a radiochemical purity of higher than 95% (RTLC). The average chelate to antibody ratio (c/a) for the conjugate used in this study was 5.8: 1. The final compound was stable in presence of PBS at 370C and room temperature. The sample was showed to have similar patterns of migration in the gel electrophoresis similar to the native protein. The accumulation of the radiolabeled antibody in liver, spleen, kidney, heart and other tissues demonstrates.67Ga-DOTA-Trastuzumab was prepared as a surrogate for important clinically applicable radionuclides used in SPECT and PET including In-111 and Cu-64 as a model of RADIOLABELING. It is also a potential compound for molecular imaging of SPECT for diagnosis and treatment studies and follow-up of HER2 expression in oncology.

Cites

  • No record.

    • References

  • No record.

    • Cite

    APA: Copy

    ALIREZAPOUR, BEHROOZ, JALILIAN, AMIR R., BOLOURINOVIN, FATEMEH, & MORADKHANI, SEDIGHEH. (2013). PRODUCTION AND QUALITY CONTROL OF [67GA]-DOTA-TRASTUZUMAB FOR RADIOIMMUNOSCINTIGRAPHY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), 12(2), 355-366. SID. https://sid.ir/paper/288157/en

    Vancouver: Copy

    ALIREZAPOUR BEHROOZ, JALILIAN AMIR R., BOLOURINOVIN FATEMEH, MORADKHANI SEDIGHEH. PRODUCTION AND QUALITY CONTROL OF [67GA]-DOTA-TRASTUZUMAB FOR RADIOIMMUNOSCINTIGRAPHY. IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR)[Internet]. 2013;12(2):355-366. Available from: https://sid.ir/paper/288157/en

    IEEE: Copy

    BEHROOZ ALIREZAPOUR, AMIR R. JALILIAN, FATEMEH BOLOURINOVIN, and SEDIGHEH MORADKHANI, “PRODUCTION AND QUALITY CONTROL OF [67GA]-DOTA-TRASTUZUMAB FOR RADIOIMMUNOSCINTIGRAPHY,” IRANIAN JOURNAL OF PHARMACEUTICAL RESEARCH (IJPR), vol. 12, no. 2, pp. 355–366, 2013, [Online]. Available: https://sid.ir/paper/288157/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top
    telegram sharing button
    whatsapp sharing button
    linkedin sharing button
    twitter sharing button
    email sharing button
    email sharing button
    email sharing button
    sharethis sharing button