GENETIC DIVERSITY IN IRANIAN ONION LANDRACES USING RAPD MARKERS

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MOUSAVIZADEH S.A.; TOURCHI MAHMOUD; MOGHADAM MOHAMMAD; MOHAMMADI S.A.; MASIHA S.

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Journal: JOURNAL OF AGRICULTURAL KNOWLEDGE Year:2006 | Volume:16 | Issue:1 Page(s): 265-272

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Title

GENETIC DIVERSITY IN IRANIAN ONION LANDRACES USING RAPD MARKERS

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Keywords

A MOVAQ3

CLUSTER ANALYSISQ3

GENETIC DIVERSITYQ2

ONIONQ2

PRINCIPAL COORDINATE ANALYSISQ2

RAPDQ3

Abstract

In order to evaluate GENETIC DIVERSITY within and among Iranian ONION landraces, twenty ONION landraces along with two hybrids were screened using random amplified polymorphic DNA (RAPD) markers. Twenty- six out of 200 tested RAPD primers produced 170 polymorphic bands. The total number of polymorphic bands within landraces and hybrids varied from 66 to 131 and genetic distances of entries based on Nei's formula ranged from 0.037 to 0.31. The averages of total and within population GENETIC DIVERSITY of landraces and hybrids were 0.341 and 0.234, respectively, and the degree of genetic differentiation was estimated 0.31. Using Nei's gene index, gene diversity within landraces and hybrids was estimated in the range of zero to 0.5. Analysis of molecular variance (AMOVA) based on squared Euclidean distances showed significant variance within and among ONION entries, but the between entry variance (%25.7) was less than that of within-entry variance (%74.3). CLUSTER ANALYSIS grouped the entries into four clusters. Molecular grouping showed no correspondence with the geographical pattern. PRINCIPAL COORDINATE ANALYSIS showed that the first three coordinates explained %52.29 of the total variations, indicating suitable genomic distribution for the markers under study.

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APA: Copy

MOUSAVIZADEH, S.A., TOURCHI, MAHMOUD, MOGHADAM, MOHAMMAD, MOHAMMADI, S.A., & MASIHA, S.. (2006). GENETIC DIVERSITY IN IRANIAN ONION LANDRACES USING RAPD MARKERS. JOURNAL OF AGRICULTURAL KNOWLEDGE, 16(1), 265-272. SID. https://sid.ir/paper/28899/en

Vancouver: Copy

MOUSAVIZADEH S.A., TOURCHI MAHMOUD, MOGHADAM MOHAMMAD, MOHAMMADI S.A., MASIHA S.. GENETIC DIVERSITY IN IRANIAN ONION LANDRACES USING RAPD MARKERS. JOURNAL OF AGRICULTURAL KNOWLEDGE[Internet]. 2006;16(1):265-272. Available from: https://sid.ir/paper/28899/en

IEEE: Copy

S.A. MOUSAVIZADEH, MAHMOUD TOURCHI, MOHAMMAD MOGHADAM, S.A. MOHAMMADI, and S. MASIHA, “GENETIC DIVERSITY IN IRANIAN ONION LANDRACES USING RAPD MARKERS,” JOURNAL OF AGRICULTURAL KNOWLEDGE, vol. 16, no. 1, pp. 265–272, 2006, [Online]. Available: https://sid.ir/paper/28899/en

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