IDENTIFICATION OF BIFIDOBACTERIUM STRAINS ISOLATED FROM FECAL SAMPLES OF SOME IRANIAN SUBJECTS USING 16SRRNA GENE SEQUENCE ANALYSIS AND PCR-BASED GENE SPECIFIC PRIMERS

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KHOMEYRI M.; MORTAZAVI S.A.A.GH.; GHODOUSI H.B.; SHAHIDI F.; KHAMESAN A.; AHMAD D.

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Paper Information

Journal: Journal of Kerman University of Medical Sciences Year:2005 | Volume:12 | Issue:1 Page(s): 21-31

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Title

IDENTIFICATION OF BIFIDOBACTERIUM STRAINS ISOLATED FROM FECAL SAMPLES OF SOME IRANIAN SUBJECTS USING 16SRRNA GENE SEQUENCE ANALYSIS AND PCR-BASED GENE SPECIFIC PRIMERS

Author(s)

Keywords

BIFIDOBACTERIUMQ2

16S RRNAQ4

PCRQ2

IRANIAN STRAINSQ2

BIF 164FQ4

BIF601RQ2

IDENTIFICATIONQ2

Abstract

For the first time in Iran 40 strains of BIFIDOBACTERIUM were isolated from feces of Iranian subjects. By using phenotypic tests, 18 isolates were identified as BIFIDOBACTERIUM longum, 10 as BIFIDOBACTERIUM bifidum and one as BIFIDOBACTERIUM catlinolatum. In order to validate these results and also to IDENTIFICATIONs other isolates that had not been identified by phenotypic tests, two methods of PCR with genus-specific primers of Bif164f and BIF601R and 16SrRNA gene sequence analysis were applied. Results of PCR confirmed the obtained phenotypic IDENTIFICATIONs. Moreover by this method the 8 remaining strains were identified as BIFIDOBACTERIUM species. Using sequencing 16SrRNA gene, 5 B. longum strains were identified that had different fermentation pattern from B. longum. Some new ribose negative BIFIDOBACTERIUM longum strains were also identified. The obtained results present new strains of BIFIDOBACTERIUM longum.

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References

ISOLATION, IDENTIFICATION AND DISTRIBUTION OF BIFIDOBACTERIUM SPP. IN SOME IRANIAN SUBJECTS

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APA: Copy

KHOMEYRI, M., MORTAZAVI, S.A.A.GH., GHODOUSI, H.B., SHAHIDI, F., KHAMESAN, A., & AHMAD, D.. (2005). IDENTIFICATION OF BIFIDOBACTERIUM STRAINS ISOLATED FROM FECAL SAMPLES OF SOME IRANIAN SUBJECTS USING 16SRRNA GENE SEQUENCE ANALYSIS AND PCR-BASED GENE SPECIFIC PRIMERS. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, 12(1), 21-31. SID. https://sid.ir/paper/290/en

Vancouver: Copy

KHOMEYRI M., MORTAZAVI S.A.A.GH., GHODOUSI H.B., SHAHIDI F., KHAMESAN A., AHMAD D.. IDENTIFICATION OF BIFIDOBACTERIUM STRAINS ISOLATED FROM FECAL SAMPLES OF SOME IRANIAN SUBJECTS USING 16SRRNA GENE SEQUENCE ANALYSIS AND PCR-BASED GENE SPECIFIC PRIMERS. JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES[Internet]. 2005;12(1):21-31. Available from: https://sid.ir/paper/290/en

IEEE: Copy

M. KHOMEYRI, S.A.A.GH. MORTAZAVI, H.B. GHODOUSI, F. SHAHIDI, A. KHAMESAN, and D. AHMAD, “IDENTIFICATION OF BIFIDOBACTERIUM STRAINS ISOLATED FROM FECAL SAMPLES OF SOME IRANIAN SUBJECTS USING 16SRRNA GENE SEQUENCE ANALYSIS AND PCR-BASED GENE SPECIFIC PRIMERS,” JOURNAL OF KERMAN UNIVERSITY OF MEDICAL SCIENCES, vol. 12, no. 1, pp. 21–31, 2005, [Online]. Available: https://sid.ir/paper/290/en

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