ANALYSIS OF GUS GENE INHERITANCE AND CAMV 35S PROMOTER ACTIVITY IN SECOND
GENERATION OF TRANSFORMED CANOLA

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

MIRZAGHADERI GH.; JALALI JAVARAN MOKHTAR; SHAKIB A.M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: JOURNAL OF AGRICULTURAL KNOWLEDGE Year:2004 | Volume:14 | Issue:2 Page(s): 95-111

Download Full-Text

Persian Verion

View:

940

Download:

Cites:

Information Journal Paper

Title

ANALYSIS OF GUS GENE INHERITANCE AND CAMV 35S PROMOTER ACTIVITY IN SECOND GENERATION OF TRANSFORMED CANOLA

Author(s)

Keywords

CAMV 35S PROMOTERQ2

CANOLAQ2

GUS GENEQ3

REPORTER GENEQ2

TRANSGENIC PLANTQ2

Abstract

The GUS GENE was used to assess CAMV 35S PROMOTER activity and to optimize an efficient transformation system for CANOLA (Brassica napus). Second generation of transformed CANOLA plants containing kanamycin resistant (KanR) and GUS GENE linked to the CAMV 35S PROMOTER were used to histochemically analysis the 35S promoter functions. The GUS inheritance was tested using PCR technique and kanamycin resistant gene. The majority of the seedlings (more than 70%) were kanamycin resistant. Plant segregation pattern for kanamycin resistance revealed that some of the transformants contained more than one T-DNA. Tissue samples from different parts of TRANSGENIC PLANTs including callus, shoots and flowers were tested by histochemical analysis. The GUS GENE. controlled by 35S promoter was expressed continuously in different parts of the TRANSGENIC PLANTs as well as during different developmental stages (before organogenesis, after regeneration and during reproduction). The GUS GENE expression studies showed that CAMV 35S PROMOTER is a strong promoter which can express the transformed gene in different tissues at different developmental stages in CANOLA.

Cites

GENETIC ANALYSIS OF CALLUS INDUCTION AND GROWTH IN RAPESEED

References

No record.

Cite

APA: Copy

MIRZAGHADERI, GH., JALALI JAVARAN, MOKHTAR, & SHAKIB, A.M.. (2004). ANALYSIS OF GUS GENE INHERITANCE AND CAMV 35S PROMOTER ACTIVITY IN SECOND GENERATION OF TRANSFORMED CANOLA. JOURNAL OF AGRICULTURAL KNOWLEDGE, 14(2), 95-111. SID. https://sid.ir/paper/29009/en

Vancouver: Copy

MIRZAGHADERI GH., JALALI JAVARAN MOKHTAR, SHAKIB A.M.. ANALYSIS OF GUS GENE INHERITANCE AND CAMV 35S PROMOTER ACTIVITY IN SECOND GENERATION OF TRANSFORMED CANOLA. JOURNAL OF AGRICULTURAL KNOWLEDGE[Internet]. 2004;14(2):95-111. Available from: https://sid.ir/paper/29009/en

IEEE: Copy

GH. MIRZAGHADERI, MOKHTAR JALALI JAVARAN, and A.M. SHAKIB, “ANALYSIS OF GUS GENE INHERITANCE AND CAMV 35S PROMOTER ACTIVITY IN SECOND GENERATION OF TRANSFORMED CANOLA,” JOURNAL OF AGRICULTURAL KNOWLEDGE, vol. 14, no. 2, pp. 95–111, 2004, [Online]. Available: https://sid.ir/paper/29009/en

Related Journal Papers