INVESTIGATION OF CALPASTATIN GENE POLYMORPHISM AND ITS RELATIONSHIP WITH DAILY GAIN IN IRANIAN GHEZEL SHEEP

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MAHDAVI MAMAGHANI A.; SHOJA GHIAS JALIL; PIRANI N.A.; SHEYKHLOU M.R.

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Journal: JOURNAL OF AGRICULTURAL KNOWLEDGE Year:2009 | Volume:18 | Issue:4 Page(s): 163-170

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Title

INVESTIGATION OF CALPASTATIN GENE POLYMORPHISM AND ITS RELATIONSHIP WITH DAILY GAIN IN IRANIAN GHEZEL SHEEP

Author(s)

MAHDAVI MAMAGHANI A. | SHOJA GHIAS JALIL | PIRANI N.A. | SHEYKHLOU M.R. | Issue Writer Certificate

Keywords

CALPASTATINQ3

PCR-SSCPQ2

GHEZEL SHEEPQ2

HETEROZYGOSITYQ3

Abstract

Polymorphism of CAST gene was investigated with 200 purebred GHEZEL SHEEP which were randomly collected from Ghezel breeding station of Myandoab. After extracting of genomic DNA, exon 1 of CALPASTATIN gene with 622 bp was amplified with specific primers. The SSCP technique was used for detecting the PCR products using polyacrilamid gel and silver staining method. In this research three alleles were observed having the frequencies of 0.62, 0.35 and 0.03, respectively. Comparison of allele frequencies indicated that Ghezel population was not in Hardy-Weinberg equilibrium. Average HETEROZYGOSITY for GHEZEL SHEEP was 37%. The result 'also indicated that genotype had a significant effect on daily gain from birth to six month's weight (P£0.05). Therefore, the results confirmed that polymorphism at this gene can be a useful tool for selection programs based on marker-assisted selection.

Cites

References

STUDY OF CALPAIN GENE POLYMORPHISM AND ITS ASSOCIATION TO DAILY GAIN IN BALUCHI SHEEP

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APA: Copy

MAHDAVI MAMAGHANI, A., SHOJA GHIAS, JALIL, PIRANI, N.A., & SHEYKHLOU, M.R.. (2009). INVESTIGATION OF CALPASTATIN GENE POLYMORPHISM AND ITS RELATIONSHIP WITH DAILY GAIN IN IRANIAN GHEZEL SHEEP. JOURNAL OF AGRICULTURAL KNOWLEDGE, 18(4), 163-170. SID. https://sid.ir/paper/29049/en

Vancouver: Copy

MAHDAVI MAMAGHANI A., SHOJA GHIAS JALIL, PIRANI N.A., SHEYKHLOU M.R.. INVESTIGATION OF CALPASTATIN GENE POLYMORPHISM AND ITS RELATIONSHIP WITH DAILY GAIN IN IRANIAN GHEZEL SHEEP. JOURNAL OF AGRICULTURAL KNOWLEDGE[Internet]. 2009;18(4):163-170. Available from: https://sid.ir/paper/29049/en

IEEE: Copy

A. MAHDAVI MAMAGHANI, JALIL SHOJA GHIAS, N.A. PIRANI, and M.R. SHEYKHLOU, “INVESTIGATION OF CALPASTATIN GENE POLYMORPHISM AND ITS RELATIONSHIP WITH DAILY GAIN IN IRANIAN GHEZEL SHEEP,” JOURNAL OF AGRICULTURAL KNOWLEDGE, vol. 18, no. 4, pp. 163–170, 2009, [Online]. Available: https://sid.ir/paper/29049/en

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