MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS

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ZARNANI A.H.; MOAZENI S.M.; BAYAT A.A.; MAHMOODI S.A.R.; JEDI TEHRANI MAHMOUD; DOUKOUHAKI POUNEH; SALEHNIA M.

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2003 | Volume:2 | Issue:3 Page(s): 139-144

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Title

MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS

Author(s)

Keywords

DENDRITIC CELL

CYTOCHEMISTRY

MORPHOLOGY

MYELOPEROXIDASE

NONSPECIFIC ESTERASE

Abstract

DENDRITIC CELLs function as the main cellular population responsible for professional antigen presentation and hence for induction of primary immune responses. Although they are present in virtually every tissue, nevertheless their number is usually so low that it makes their isolation for studies very difficult. In this study, we purified DENDRITIC CELLs from mouse spleen by a three-step enrichment method and evaluated morphological and cytochemical characteristics of isolated cells. We showed that isolated DENDRITIC CELLs from mouse spleen had all lobulated nuclei with multiple cytoplasmic projections and their morphological features changed after an overnight incubation. It was also shown that typical DENDRITIC CELLs lacked both MYELOPEROXIDASE (MPO) and Non Specific Esterase (NSE) activity. In conclusion, for reaching a reasonable purity in isolation of DENDRITIC CELLs from lymphoid tissues, many enrichment steps should be taken, and for determining the purity of isolated cells, we recommend that a combination of morphological and cytochemical studies be used.

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APA: Copy

ZARNANI, A.H., MOAZENI, S.M., BAYAT, A.A., MAHMOODI, S.A.R., JEDI TEHRANI, MAHMOUD, DOUKOUHAKI, POUNEH, & SALEHNIA, M.. (2003). MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS . IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 2(3), 139-144. SID. https://sid.ir/paper/291030/en

Vancouver: Copy

ZARNANI A.H., MOAZENI S.M., BAYAT A.A., MAHMOODI S.A.R., JEDI TEHRANI MAHMOUD, DOUKOUHAKI POUNEH, SALEHNIA M.. MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS . IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2003;2(3):139-144. Available from: https://sid.ir/paper/291030/en

IEEE: Copy

A.H. ZARNANI, S.M. MOAZENI, A.A. BAYAT, S.A.R. MAHMOODI, MAHMOUD JEDI TEHRANI, POUNEH DOUKOUHAKI, and M. SALEHNIA, “MORPHOLOGICAL AND CYTOCHEMICAL CHARACTERISTICS OF PURIFIED MURINE SPLENIC DENDRITIC CELLS ,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 2, no. 3, pp. 139–144, 2003, [Online]. Available: https://sid.ir/paper/291030/en

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