COMPARISON OF TWO PROLIFERATION ASSAYSMTT ANDLTT IN IMMUNODEFICIENT PATIENTS

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ISAIAN A.; POURPAK Z.; NIKFARJAM L.; FARHOUDI A.

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Journal Paper

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Journal: Iranian Journal of Allergy, Asthma and Immunology Year:2003 | Volume:2 | Issue:2 Page(s): 0-0

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Title

COMPARISON OF TWO PROLIFERATION ASSAYSMTT ANDLTT IN IMMUNODEFICIENT PATIENTS

Author(s)

ISAIAN A. | POURPAK Z. | NIKFARJAM L. | FARHOUDI A. | Issue Writer Certificate

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Abstract

MTT assay is designed for spectrophotometric quantification of cell growth and viability without using the radiactive isotopes. The aim of this study is comparison of LTT and MTT as sensitive poliferation assays in immunodeficient patients. 20 immunodeficient and 20 healthy subjects were selected in this study. All of them had normal lymphocytes count, normal CD3 and negative DTH reaction to PPD, DT and candida. The immunodeficient patients regularly suffered from herpes, candida and staph abscess. The lymphocytes of immunodeficient control groups were treated with PHA as mitogen. The lymphocyte proliferation was evaluated by MIT and LTT. The results showed a strong correlation between LTT and MITT between immunodeficient patients and healthy controls. The sensitivity test for MTT was 90%, and the sensitivity test for LTT was 98%. MTI method can be considered as a non-radiactive evaluation of cell proliferation. Detecting cell proliferation with accurate, sensitive, fast, easy and safe method is more preferable than LIT.

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APA: Copy

ISAIAN, A., POURPAK, Z., NIKFARJAM, L., & FARHOUDI, A.. (2003). COMPARISON OF TWO PROLIFERATION ASSAYSMTT ANDLTT IN IMMUNODEFICIENT PATIENTS. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), 2(2), 0-0. SID. https://sid.ir/paper/291074/en

Vancouver: Copy

ISAIAN A., POURPAK Z., NIKFARJAM L., FARHOUDI A.. COMPARISON OF TWO PROLIFERATION ASSAYSMTT ANDLTT IN IMMUNODEFICIENT PATIENTS. IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI)[Internet]. 2003;2(2):0-0. Available from: https://sid.ir/paper/291074/en

IEEE: Copy

A. ISAIAN, Z. POURPAK, L. NIKFARJAM, and A. FARHOUDI, “COMPARISON OF TWO PROLIFERATION ASSAYSMTT ANDLTT IN IMMUNODEFICIENT PATIENTS,” IRANIAN JOURNAL OF ALLERGY, ASTHMA AND IMMUNOLOGY (IJAAI), vol. 2, no. 2, pp. 0–0, 2003, [Online]. Available: https://sid.ir/paper/291074/en

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