DETECTION AND CHARACTERIZATION OF A NOVEL LYTIC BACTERIOPHAGE (VB-KPNEM-ISF48) AGAINST KLEBSIELLA PNEUMONIAE ISOLATES FROM INFECTED WOUNDS CARRYING ANTIBIOTIC-RESISTANCE GENES (TEM, SHV, AND CTX-M)

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KOMIJANI MAJID; BOUZARI MAJID; RAHIMI FATEH

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Journal: Iranian Red Crescent Medical Journal Year:2017 | Volume:19 | Issue:2 Page(s): 0-0

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Title

DETECTION AND CHARACTERIZATION OF A NOVEL LYTIC BACTERIOPHAGE (VB-KPNEM-ISF48) AGAINST KLEBSIELLA PNEUMONIAE ISOLATES FROM INFECTED WOUNDS CARRYING ANTIBIOTIC-RESISTANCE GENES (TEM, SHV, AND CTX-M)

Author(s)

KOMIJANI MAJID | BOUZARI MAJID | RAHIMI FATEH | Issue Writer Certificate

Keywords

ANTIBIOTIC RESISTANCE

BACTERIOPHAGE

KLEBSIELLA PNEUMONIAE

Abstract

Background: Approximately 80% of nosocomial infections are caused by strains of KLEBSIELLA PNEUMONIAE. Resistance to b-lactam antibiotics is a result of expression of extended-spectrum b-lactamase (ESBL) genes. Recently, phage therapy has gained increasing attention due to its many advantages over chemotherapy.Objectives: The aim of this study was to isolate ESBL-positive KLEBSIELLA PNEUMONIAE strains from different types of wounds, and a lytic BACTERIOPHAGE against them.Methods: During a two-year period from January 2013 to February 2015, in a cross-sectional study, 41 K. pneumoniae strains were isolated from 193 categories of infected wounds at three hospitals in Isfahan, Iran. Phenotypic and genotypic methods were used to detect the ESBL-positive strains. A lytic phage against K. pneumoniae was isolated, and its host range, morphology, thermal and pH stability, saline stress, and estimated genome size were determined.Results: Of the 41 K. pneumoniae isolates, 18 were ESBL-producing and 36 carried antibiotic-resistance genes. A total of 36 out of 41 isolated samples carried one or more resistance genes. The results showed that the differences between phenotypic and genotypic identification methods were significant (P=0.0001). The SHV, CTX-M, and TEM genes were detected in 29, 10, and 9 isolates of the tested bacteria, respectively. No bacteria contained both the SHV and the CTX-M genes. The frequency of the SHV gene was significantly higher than that of the other genes (P=0.0001). The phage’s morphology features placed it in the Myoviridae family. Only 38 out of 41 clinical isolates were susceptible to the phage. Phage titers were completely preserved after one hour of incubation at 30oC and 40oC, and they were stable at different pH values. The phage’s survival decreased when the salt concentration was increased.Conclusions: The high rate of isolation of antibiotic-resistant strains of K. pneumoniae was consistent with other studies. As the phage was virulent and specific for K. pneumoniae, and was stable and active at different pH values, salt concentrations, and temperatures, its application in phage therapy of infected wounds is suggested.

Cites

Isolation and Characterization of a Klebsiella pneumoniae Specific Lytic Bacteriophage from a Hospital Waste-water Treatment Plant

References

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APA: Copy

KOMIJANI, MAJID, BOUZARI, MAJID, & RAHIMI, FATEH. (2017). DETECTION AND CHARACTERIZATION OF A NOVEL LYTIC BACTERIOPHAGE (VB-KPNEM-ISF48) AGAINST KLEBSIELLA PNEUMONIAE ISOLATES FROM INFECTED WOUNDS CARRYING ANTIBIOTIC-RESISTANCE GENES (TEM, SHV, AND CTX-M). IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), 19(2), 0-0. SID. https://sid.ir/paper/293336/en

Vancouver: Copy

KOMIJANI MAJID, BOUZARI MAJID, RAHIMI FATEH. DETECTION AND CHARACTERIZATION OF A NOVEL LYTIC BACTERIOPHAGE (VB-KPNEM-ISF48) AGAINST KLEBSIELLA PNEUMONIAE ISOLATES FROM INFECTED WOUNDS CARRYING ANTIBIOTIC-RESISTANCE GENES (TEM, SHV, AND CTX-M). IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ)[Internet]. 2017;19(2):0-0. Available from: https://sid.ir/paper/293336/en

IEEE: Copy

MAJID KOMIJANI, MAJID BOUZARI, and FATEH RAHIMI, “DETECTION AND CHARACTERIZATION OF A NOVEL LYTIC BACTERIOPHAGE (VB-KPNEM-ISF48) AGAINST KLEBSIELLA PNEUMONIAE ISOLATES FROM INFECTED WOUNDS CARRYING ANTIBIOTIC-RESISTANCE GENES (TEM, SHV, AND CTX-M),” IRANIAN RED CRESCENT MEDICAL JOURNAL (IRCMJ), vol. 19, no. 2, pp. 0–0, 2017, [Online]. Available: https://sid.ir/paper/293336/en

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