TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637

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SOHEYLI Z.S.; GOLIAEI B.

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Journal: Medical Journal of The Islamic Republic of Iran Year:2002 | Volume:15 | Issue:4 Page(s): 219-225

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Title

TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637

Author(s)

SOHEYLI Z.S. | GOLIAEI B. | Issue Writer Certificate

Keywords

TGF-ß

GM-CSF

HTB-5637

BLADDER CARCINOMA

Abstract

Transforming growth factor betas are multifunctional polypeptides in the cytokine superfamily. They have a growth inhibitory role on hemopoietic progenitor cells in semisolid colony assay as well as in long-term bone-marrow culture. TGF-ß2 represses stromal cells, stem cell factor gene transcription, and decreases the stability of c-kit transcripts in hemopoietic cells. TGF- ß also modulates GM-CSF production from human lymphocytes. The present study reveals the TGF- ß2 role in production of GM-CSF in HTB 5637, human BLADDER CARCINOMA cell line. HTB 5637 cells were treated with 5 ng/mL of human TGF- ß2 viable cells were counted and GM-CSF concentrationwas determined. No antiproliferate activity of TGF- ß2 on HTB 5637 cell line was observed. Biological assay showed increased levels of GM-CSF in the supernatant of cultured cells. However this increase was lower than that expected from ELISA. Since TGF- ß may be an active suppressor factor regulating hemopoiesis, it seems that some inhibitory factor(s) may be produced (increased) in response to TGF- ß2 treatment. It has been shown that GM-CSF mRNA content from HTB 5637 cell line is very stable and this stabilization is translational dependent. Using Slot blot and Northern blot analysis, we determined that TGF- ß2 upregulated GM-CSF gene expression in HTB 5637 cell line. The results suggest that TGF- ß2 upregulates the production of GM-CSF gene at the transcriptional level.

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APA: Copy

SOHEYLI, Z.S., & GOLIAEI, B.. (2002). TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637. MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI), 15(4), 219-225. SID. https://sid.ir/paper/294069/en

Vancouver: Copy

SOHEYLI Z.S., GOLIAEI B.. TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637. MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)[Internet]. 2002;15(4):219-225. Available from: https://sid.ir/paper/294069/en

IEEE: Copy

Z.S. SOHEYLI, and B. GOLIAEI, “TRANSFORMING GROWTH FACTOR ß2 UP-REGULATES GM-CSF GENE IN HUMAN BLADDER CARCINOMA CELL LINE HTB 5637,” MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI), vol. 15, no. 4, pp. 219–225, 2002, [Online]. Available: https://sid.ir/paper/294069/en

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