A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATASE ACTIVITY FOR MEASURING CELLULAR FUNCTIONS

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DABARE A.A.N.P.M.; NOURI A.M.E.; OLIVER R.T.D.

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Paper Information

Journal: Medical Journal of The Islamic Republic of Iran Year:2000 | Volume:14 | Issue:1 Page(s): 53-59

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Title

A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATASE ACTIVITY FOR MEASURING CELLULAR FUNCTIONS

Author(s)

DABARE A.A.N.P.M. | NOURI A.M.E. | OLIVER R.T.D. | Issue Writer Certificate

Keywords

ALKALINE PHOSPHATASES

TESTIS TUMOR

CELL PROLIFERATION

Abstract

In this study cellular phosphates activity, referred to as enzyme developed color (EDC) assay, was used for an in vitro assessment of CELL PROLIFERATION and cytotoxicity. Optimum conditions were established and found to be 10x103 cells / well at 37°C and 60 minincubation (for developing color).Under the same conditions a direct correlation between optical density (OD) and cell number was observed. Thus, the aDs for a cell line, Ish at 100, 50, 25 and 10 X103 cells/well were 1.08±0.05, 0.59±0.02, 0.33±0.02, and 0.17 ±0.0 1 respectively (r=0.995, p<0.001). When EDC was compared with another colorimetric assay, i.e. MTf, the results showed a direct correlation with r=0.995, p<0.001. Repeating the experiment with live and fixed tumor cells showed similar results. Thus, for the Ish line, the aDs at 50 and 10 X103 cells/well for fixed and live cells were 0.66:t0.Ol, 0.18±0.01 and , 0.64±0.03 (p>0.05), 0.19±0.01 (p>0.05), respectively. The assay was also shown to be suitable for the measurement of cell cytotoxicity and compared well with the MTT assay.These findings indicated that a simple, rapid and economical EDC assay could be used to investigate various cellular functions. The main advantage of EDC is it's suitability touse stored cells. This provides flexibility for testing samples stored over a long period in order to limit inter-experimental variations.

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APA: Copy

DABARE, A.A.N.P.M., NOURI, A.M.E., & OLIVER, R.T.D.. (2000). A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATASE ACTIVITY FOR MEASURING CELLULAR FUNCTIONS. MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI), 14(1), 53-59. SID. https://sid.ir/paper/294303/en

Vancouver: Copy

DABARE A.A.N.P.M., NOURI A.M.E., OLIVER R.T.D.. A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATASE ACTIVITY FOR MEASURING CELLULAR FUNCTIONS. MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI)[Internet]. 2000;14(1):53-59. Available from: https://sid.ir/paper/294303/en

IEEE: Copy

A.A.N.P.M. DABARE, A.M.E. NOURI, and R.T.D. OLIVER, “A NEW CYTOCOLORIMETRIC ASSAY USING PHOSPHATASE ACTIVITY FOR MEASURING CELLULAR FUNCTIONS,” MEDICAL JOURNAL OF THE ISLAMIC REPUBLIC OF IRAN (MJIRI), vol. 14, no. 1, pp. 53–59, 2000, [Online]. Available: https://sid.ir/paper/294303/en

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