DETECTING THE ANOPHELES CULICIFACIES COMPLEX, SPECIES A AND B IN BALUCHESTAN USING MT DNA PCR-RFLP ASSAY; THE FIRST REPORT OF SPECIES B FROM IRAN

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OSHAGHI M.A.; TAGHILOU B.; MORADI M.T.; VATANDOUST H.

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Journal: Hakim Journal Year:2004 | Volume:7 | Issue:1 Page(s): 35-41

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Title

DETECTING THE ANOPHELES CULICIFACIES COMPLEX, SPECIES A AND B IN BALUCHESTAN USING MT DNA PCR-RFLP ASSAY; THE FIRST REPORT OF SPECIES B FROM IRAN

Author(s)

OSHAGHI M.A. | TAGHILOU B. | MORADI M.T. | VATANDOUST H. | Issue Writer Certificate

Keywords

ANOPHELES CULICIFACIES COMPLEXQ3

SPECIES AQ4

SPECIES BQ4

PCR-RFLPQ4

MTDNAQ4

CYTOCHROME OXIDASE SUBUNIT I AND IL (COI-II)Q4

BALUCHESTANQ3

IRANQ3

Abstract

Introduction: Mosquitoes of the ANOPHELES CULICIFACIES COMPLEX are one of the main malaria vectors in Indian subcontinent and Middle East countries as well as in IRAN. Based on cytological characteristics there are five morphologically indistinguishable species in this complex that called A, B, C, D and E. These species have got different biological and behaviour characteristics, and show different vectorial capacity. Methods: Due to problems inherent in the cytological studies a DNA based method MTDNA PCR-RFLP was conducted to study genetic variation and it's utility in identifying species composition of the complex in BALUCHESTAN district of IRAN. A 1512 bp MTDNA of cytochrome oxidase subunit I and II (COl-COIl) or an 875bp MTDNA of COl were amplified and treated by different restriction enzymes (PCR-RFLP). Results: Results of this study showed that at least two species, A and B, of the complex are present in the region. This is the first report on the occurrence of SPECIES B in IRAN. In addition to SPECIES A and B, there were some other haplotypes within the specimens which represent either the occurrence of other members of the complex (presumably species E) or interspecies variation. Conclusion: These results are very important in epidemiology of malaria in the region and will be remain to be confirmed by further cytological and molecular works.

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APA: Copy

OSHAGHI, M.A., TAGHILOU, B., MORADI, M.T., & VATANDOUST, H.. (2004). DETECTING THE ANOPHELES CULICIFACIES COMPLEX, SPECIES A AND B IN BALUCHESTAN USING MT DNA PCR-RFLP ASSAY; THE FIRST REPORT OF SPECIES B FROM IRAN. HAKIM RESEARCH JOURNAL, 7(1), 35-41. SID. https://sid.ir/paper/29480/en

Vancouver: Copy

OSHAGHI M.A., TAGHILOU B., MORADI M.T., VATANDOUST H.. DETECTING THE ANOPHELES CULICIFACIES COMPLEX, SPECIES A AND B IN BALUCHESTAN USING MT DNA PCR-RFLP ASSAY; THE FIRST REPORT OF SPECIES B FROM IRAN. HAKIM RESEARCH JOURNAL[Internet]. 2004;7(1):35-41. Available from: https://sid.ir/paper/29480/en

IEEE: Copy

M.A. OSHAGHI, B. TAGHILOU, M.T. MORADI, and H. VATANDOUST, “DETECTING THE ANOPHELES CULICIFACIES COMPLEX, SPECIES A AND B IN BALUCHESTAN USING MT DNA PCR-RFLP ASSAY; THE FIRST REPORT OF SPECIES B FROM IRAN,” HAKIM RESEARCH JOURNAL, vol. 7, no. 1, pp. 35–41, 2004, [Online]. Available: https://sid.ir/paper/29480/en

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