THE ASSESSMENT OF UNIVERSAL PRIMER FOR RAPID DETECTION OF BACTERIAL MENINGITIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ATAEI R.A.; MEHRABI TAVANA A.; KARAMI ALI; IZADI MORTEZA; HASANI S.M.J.; SAFIRI Z.; ALAHVERDI M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Hakim Journal Year:2009 | Volume:11 | Issue:4 Page(s): 27-32

Download Full-Text

Persian Verion

View:

1,252

Download:

Cites:

Information Journal Paper

Title

THE ASSESSMENT OF UNIVERSAL PRIMER FOR RAPID DETECTION OF BACTERIAL MENINGITIS

Author(s)

Keywords

BACTERIAL MENINGITISQ3

DIAGNOSISQ3

PRIMER 16S RRNAQ3

PCRQ3

Abstract

Introduction: The aim of this study was to design a molecular detection for differentiation of bacterial from nonBACTERIAL MENINGITIS.Methods: In this research, the universal primer was used. The DNA of 20 bacterial strains was extracted. Eubacterial 16SrRNA gene fragments (16S rDNA) were amplified by PCR with broad-range ribosomal primers. The PCR was carried out under optimized standard conditions. The PCR products were electrophoresed on agarose 1 % and compared with standard strains. The high specificity of the PCR assay was documented after testing bacteria of 20 different species.Results: At least 10 bacteria could be found in 1 ml of fluid culture. The use of universal primer which contains 16SrRNA could be suitable amplification of a sequence with 1000bp. This sequence was found very similar in nearly all bacteria. Twenty different bacterial strains were tested. The sequence (1000bp) was seen in all bacterial strains applied in this study.Conclusion: Use of this molecular method for any BACTERIAL MENINGITIS is applicable because of fast DIAGNOSIS in 3 hours, differentiating BACTERIAL MENINGITIS from non-bacterial, and finally the effective treatment of patients suffered with the disease. The method is effective in developing and developed countries.

Cites

No record.

References

No record.

Cite

APA: Copy

ATAEI, R.A., MEHRABI TAVANA, A., KARAMI, ALI, IZADI, MORTEZA, HASANI, S.M.J., SAFIRI, Z., & ALAHVERDI, M.. (2009). THE ASSESSMENT OF UNIVERSAL PRIMER FOR RAPID DETECTION OF BACTERIAL MENINGITIS. HAKIM RESEARCH JOURNAL, 11(4), 27-32. SID. https://sid.ir/paper/29520/en

Vancouver: Copy

ATAEI R.A., MEHRABI TAVANA A., KARAMI ALI, IZADI MORTEZA, HASANI S.M.J., SAFIRI Z., ALAHVERDI M.. THE ASSESSMENT OF UNIVERSAL PRIMER FOR RAPID DETECTION OF BACTERIAL MENINGITIS. HAKIM RESEARCH JOURNAL[Internet]. 2009;11(4):27-32. Available from: https://sid.ir/paper/29520/en

IEEE: Copy

R.A. ATAEI, A. MEHRABI TAVANA, ALI KARAMI, MORTEZA IZADI, S.M.J. HASANI, Z. SAFIRI, and M. ALAHVERDI, “THE ASSESSMENT OF UNIVERSAL PRIMER FOR RAPID DETECTION OF BACTERIAL MENINGITIS,” HAKIM RESEARCH JOURNAL, vol. 11, no. 4, pp. 27–32, 2009, [Online]. Available: https://sid.ir/paper/29520/en

Related Journal Papers