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Information Journal Paper

Title

EVALUATION OF TWO DNA EXTRACTION METHODS FROM MATERNAL PLASMA FOR USING IN NON-INVASIVE BOVINE FETUS GENDER DETERMINATION

Pages

  523-530

Abstract

 Background: Fetal DNA in MATERNAL PLASMA and serum has been shown to be a useful material for prenatal fetal sex determination during early gestational ages. NON-INVASIVE prenatal diagnosis is now possible at 8th week of pregnancy, by maternal blood sample testing.Objective: The purpose of this study was to evaluate two DNA EXTRACTION methods from mother plasma and its routine clinical application in BOVINE FETUS GENDER DETERMINATION with NON-INVASIVE method.Materials and Methods: Maternal blood samples were taken from 40 pregnant cows during the 8th-38th weeks of gestation. DNA was extracted from 350 ml of MATERNAL PLASMA with two salting-out and phenol-chloroform methods. The absorption in A260 and purity (A260/A280) of extracted DNA were detected by ultraviolet spectrophotometer. Three ml of the extracted DNA with phenol-chloroform method was used as a template. The PCR reaction was carried out to amplify the fragments of X and Y chromosomes of amelogenin, TSPY and BC1.2 genes.Results: The difference between the mean absorption of DNA extracted by phenol-chloroform method and salting-out method was not significant in A260 (p>0.05, p=0.3549), but the difference between mean purity (A260/A280) of DNA extracted by phenol-chloroform method and salting-out method was significant (p<0.001). X chromosome fragment was detected in all 40 samples and Y chromosome fragments were detected in 25 plasma samples which were delivered a male calf. The sensitivity and specificity of test was 100% with no false negative and false positive results.Conclusion: The results showed that phenol-chloroform method is a simple and sensitive method for isolation of fetal DNA in MATERNAL PLASMA.

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    APA: Copy

    DAVOUDI, ARASH, TARANG, ALIREZA, ALEYASIN, SEYED AHMAD, SALEHI, ABDOLREZA, SEIGHALANI, RAMIN, & TAHMORESSI, FARIDEH. (2012). EVALUATION OF TWO DNA EXTRACTION METHODS FROM MATERNAL PLASMA FOR USING IN NON-INVASIVE BOVINE FETUS GENDER DETERMINATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 10(6), 523-530. SID. https://sid.ir/paper/295934/en

    Vancouver: Copy

    DAVOUDI ARASH, TARANG ALIREZA, ALEYASIN SEYED AHMAD, SALEHI ABDOLREZA, SEIGHALANI RAMIN, TAHMORESSI FARIDEH. EVALUATION OF TWO DNA EXTRACTION METHODS FROM MATERNAL PLASMA FOR USING IN NON-INVASIVE BOVINE FETUS GENDER DETERMINATION. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2012;10(6):523-530. Available from: https://sid.ir/paper/295934/en

    IEEE: Copy

    ARASH DAVOUDI, ALIREZA TARANG, SEYED AHMAD ALEYASIN, ABDOLREZA SALEHI, RAMIN SEIGHALANI, and FARIDEH TAHMORESSI, “EVALUATION OF TWO DNA EXTRACTION METHODS FROM MATERNAL PLASMA FOR USING IN NON-INVASIVE BOVINE FETUS GENDER DETERMINATION,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 10, no. 6, pp. 523–530, 2012, [Online]. Available: https://sid.ir/paper/295934/en

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