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Information Journal Paper

Title

CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM HUMAN DENTAL PULP, PREAPICAL FOLLICLE AND PERIODONTALLIGAMENT

Pages

  234-242

Abstract

 Background: Human dental stem cells have high proliferative potential for self-renewal that is important to the regenerative capacity of the tissue.Objective: The aim was to isolate human DENTAL PULP stem cells (DPSC), PERIODONTAL LIGAMENT STEM CELLS (PDLSC) and periapical follicle stem cells (PAFSC) for their potential role in tissue regeneration.Materials and Methods: In this experimental study, the postnatal stem cells were isolated from DENTAL PULP, PREAPICAL FOLLICLE and periodontal ligament. The cells were stained for different stem cell markers by immunocytochemistry. To investigate the mesenchymal nature of cells, differentiation potential along osteoblastic and adipogenic lineages and gene expression profile were performed. For proliferation potential assay, Brdu staining and growth curve tests were performed. Finally, all three cell types were compared together regarding their proliferation, differentiation and displaying phenotype.Results: The isolated cell populations have similar fibroblastic like morphology and expressed all examined cell surface molecule markers. These cells were capable of differentiating into osteocyte with different capability and adipocyte with the same rate. PAFSCs showed more significant proliferation rate than others. Reverse transcriptase PCR (RT-PCR) for nanog, oct4, Alkaline phosphatase (ALP) and glyceraldehydes-3-phosphate dehydrogenease (GAD PH) as control gene showed strong positive expression of these genes in all three isolated cell types.Conclusion: PDLSCs, DPSCs and PAFSCs exist in various tissues of the teeth and can use as a source of MESENCHYMAL STEM CELLs for developing bioengineered organs and also in craniomaxillofacial reconstruction with varying efficiency in differentiation and proliferation.

Cites

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  • Cite

    APA: Copy

    NAVABAZAM, ALI REZA, SADEGHIAN NODOSHAN, FATEMEH, SHEIKHHA, MOHAMMAD HASAN, MIRESMAEILL, SAYYED MOHSEN, SOLEIMANI, MEHRDAD, & FESAHAR, FARZANEH. (2013). CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM HUMAN DENTAL PULP, PREAPICAL FOLLICLE AND PERIODONTALLIGAMENT. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), 11(3), 234-242. SID. https://sid.ir/paper/295957/en

    Vancouver: Copy

    NAVABAZAM ALI REZA, SADEGHIAN NODOSHAN FATEMEH, SHEIKHHA MOHAMMAD HASAN, MIRESMAEILL SAYYED MOHSEN, SOLEIMANI MEHRDAD, FESAHAR FARZANEH. CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM HUMAN DENTAL PULP, PREAPICAL FOLLICLE AND PERIODONTALLIGAMENT. INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE)[Internet]. 2013;11(3):234-242. Available from: https://sid.ir/paper/295957/en

    IEEE: Copy

    ALI REZA NAVABAZAM, FATEMEH SADEGHIAN NODOSHAN, MOHAMMAD HASAN SHEIKHHA, SAYYED MOHSEN MIRESMAEILL, MEHRDAD SOLEIMANI, and FARZANEH FESAHAR, “CHARACTERIZATION OF MESENCHYMAL STEM CELLS FROM HUMAN DENTAL PULP, PREAPICAL FOLLICLE AND PERIODONTALLIGAMENT,” INTERNATIONAL JOURNAL OF REPRODUCTIVE BIOMEDICINE (IRANIAN JOURNAL OF REPRODUCTIVE MEDICINE), vol. 11, no. 3, pp. 234–242, 2013, [Online]. Available: https://sid.ir/paper/295957/en

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