Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

video

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

sound

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Version

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

View:

185
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Download:

157
Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Cites:

Information Journal Paper

Title

Investigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria-A Preliminary Mechanistic Study

Pages

  13-24

Abstract

 Outer membrane of Gram-negative bacteria is a permeability barrier to many antibacterial agents, including the glycopeptide antibiotics such as Vancomycin hydrochloride and as a result these antibiotics are ineffective against Gram negative bacteria. Different strategies have been described to overcome such limitation, including application of nanoparticles, as was shown in our previous studies for polymeric nanoparticles. On the other hand, some nanoparticles have the ability to reduce the permeation of drugs through biological barriers. Therefore, in this investigation, the effects of Fusogenic liposomes, which are expected to interact well with biological barriers, toward antimicrobial effects of Vancomycin in different bacteria, are investigated. Vancomycin-loaded liposomes were prepared by lipid film hydration method from a phospholipid mixture composed of either DPPC: DOPE: Chol or DPPC: DOPE: CHEMS, both in 1: 0. 5: 1 molar ratios. Obtained liposomes were then assessed in regard to their antibacterial properties using broth microdilution method. Liposomes were prepared by lipid-film hydration followed by extrusion and probe sonication for size reduction. Encapsulation efficiency for large hydrophilic Vancomycin in liposomes was found to be in the range of 0. 1 to 9 % for different formulations. Probe sonicated liposomes showed smaller size and were more stable than those prepared by extrusion. Antimicrobial results showed that encapsulation of Vancomycin in liposomes decreased Antibacterial efficacy of Vancomycin and caused MIC increments, compared to those of free Vancomycin. This might indicate negligible release of this large and charged molecule from liposomes into the bacterial preplasmic space (retention of Vancomycin inside liposomal cavity or lipid-drug complexation) accompanied by inability of liposomes to permeate the bacterial barrier. Further investigations are needed to explain the interaction of liposomes with bacterial membranes.

Cites

  • No record.

    • References

    Cite

    APA: Copy

    Serri, Azadeh, Mahboubi, Arash, ZARGHI, AFSHIN, & Moghimi, Hamid R.. (2018). Investigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria-A Preliminary Mechanistic Study. IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES, 14(3), 13-24. SID. https://sid.ir/paper/300400/en

    Vancouver: Copy

    Serri Azadeh, Mahboubi Arash, ZARGHI AFSHIN, Moghimi Hamid R.. Investigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria-A Preliminary Mechanistic Study. IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES[Internet]. 2018;14(3):13-24. Available from: https://sid.ir/paper/300400/en

    IEEE: Copy

    Azadeh Serri, Arash Mahboubi, AFSHIN ZARGHI, and Hamid R. Moghimi, “Investigating the Antimicrobial Efficacy of Liposomal Vancomycin in Gram-positive and Gram-negative bacteria-A Preliminary Mechanistic Study,” IRANIAN JOURNAL OF PHARMACEUTICAL SCIENCES, vol. 14, no. 3, pp. 13–24, 2018, [Online]. Available: https://sid.ir/paper/300400/en

    Related Journal Papers

  • No record.

    • Related Seminar Papers

  • No record.

    • Related Plans

  • No record.

    • Recommended Workshops






    Move to top