DIRECT AGGLUTINATION TEST AND ENZYME LINKED IMMUNOSORBENT ASSAY WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS

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HATAM G.R.; MIKAEILI F.; SAJADI S.M.; SARKARI B.

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Journal Paper

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Journal: Iranian Journal of Parasitology Year:2007 | Volume:2 | Issue:3 Page(s): 24-28

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Title

DIRECT AGGLUTINATION TEST AND ENZYME LINKED IMMUNOSORBENT ASSAY WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS

Author(s)

HATAM G.R. | MIKAEILI F. | SAJADI S.M. | SARKARI B. | Issue Writer Certificate

Keywords

VISCERAL LEISHMANIASIS

ELISA

DAT

ANTIBODY

URINE

Abstract

Background: VISCERAL LEISHMANIASIS (VL) or Kala azar is an infectious disease caused by various species of Leishmania parasites. The aim of this study was to detect and compare the presence of anti-Leishmania antibodies in the URINE of VISCERAL LEISHMANIASIS patients using ELISA and DAT methods.Methods: A total of 30 URINE samples were collected from VL patients referred to Shiraz (southeast of Iran) hospitals. Moreover 31 URINE samples were collected from healthy individuals and patients with other diseases such as malaria, brucellosis, hydatidosis and cutaneous leishmaniasis. Collected samples were examined to detect anti-Leishmania antibodies in URINE, using ELISA and DAT.Results: Anti-Leishmania ANTIBODY was detected in URINE of 18 out of 30 (60%) VL patients by DAT while ELISA detected anti-Leishmania antibodies in URINE of 28 out of 30 (93.3%) of VL cases. Sensitivity and specificity of URINE-based DAT was 60% and 83.9%, respectively while sensitivity and specificity of URINE-based ELISA were 93.3% and 93.5%, correspondingly.Conclusion: URINE-based DAT and ELISA have a reasonable specificity and sensitivity in diagnosis of VL. Accordingly, URINE-based ELISA might be a suitable alternative for serum based assays for diagnosis of VL.

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APA: Copy

HATAM, G.R., MIKAEILI, F., SAJADI, S.M., & SARKARI, B.. (2007). DIRECT AGGLUTINATION TEST AND ENZYME LINKED IMMUNOSORBENT ASSAY WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS. IRANIAN JOURNAL OF PARASITOLOGY, 2(3), 24-28. SID. https://sid.ir/paper/303804/en

Vancouver: Copy

HATAM G.R., MIKAEILI F., SAJADI S.M., SARKARI B.. DIRECT AGGLUTINATION TEST AND ENZYME LINKED IMMUNOSORBENT ASSAY WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2007;2(3):24-28. Available from: https://sid.ir/paper/303804/en

IEEE: Copy

G.R. HATAM, F. MIKAEILI, S.M. SAJADI, and B. SARKARI, “DIRECT AGGLUTINATION TEST AND ENZYME LINKED IMMUNOSORBENT ASSAY WITH URINE SAMPLES FOR THE DIAGNOSIS OF VISCERAL LEISHMANIASIS,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 2, no. 3, pp. 24–28, 2007, [Online]. Available: https://sid.ir/paper/303804/en

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