EVALUATION OF BENZIMIDAZOLE RESISTANCE IN HAEMONCHUS CONTORTUS USING COMPARATIVE PCR-RFLPMETHODS

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NABAVI R.; SHAYAN P.; SHOKRANI H.R.; ESLAMI A.; BOKAIE S.

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Journal Paper

Paper Information

Journal: IRANIAN JOURNAL OF PARASITOLOGY Year:2011 | Volume:6 | Issue:2 Page(s): 45-53

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Title

EVALUATION OF BENZIMIDAZOLE RESISTANCE IN HAEMONCHUS CONTORTUS USING COMPARATIVE PCR-RFLPMETHODS

Author(s)

Keywords

HAEMONCHUS CONTORTUS

BENZIMIDAZOLE

DRUG RESISTANCE

PCR-RFLP

BETA TUBULIN GENE

Abstract

Background: In order to deworm the ruminants especially of sheep in Iran, consumption of BENZIMIDAZOLEs has more than 2 decades history and today farmers are using imidazothiazoles, macrocyclic lactones and mostly BENZIMIDAZOLE compounds (BZs) to treat infected farm animals.It has been demonstrated that the most common molecular mechanism leading to BZsresistance inHAEMONCHUS CONTORTUS is a single mutation at amino acid 200 (phenylalanine to tyrosine) of the isotype 1 of BETA TUBULIN GENE. According to the report of such mutations in IranianTeladorsagia circumcinctaisolates with Restriction Site Created PCR-RFLP, we decided to evaluate the frequency of such mutations inH. contortus in three different geographical areas of Iran.Methodes: A total of 102 collected adult male H. contortus were evaluated with PCR-RFLP (using PSP1406I as restriction enzyme). By means of a second step to compare function of different methods and to increase sensitivity of detection mechanism, a third of samples were examined by another PCR-RFLP method (using TaaI as restriction enzyme) and finally BETA TUBULIN GENE of two samples was sequenced.Results: All of samples were detected as BZss homozygote. Finally, BETA TUBULIN GENE sequencing of two samples showed no point mutation at codon 200.Conclusion: It seems that BZresistance of H. contortus in Iran is not a serious problem as anticipated before.

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APA: Copy

NABAVI, R., SHAYAN, P., SHOKRANI, H.R., ESLAMI, A., & BOKAIE, S.. (2011). EVALUATION OF BENZIMIDAZOLE RESISTANCE IN HAEMONCHUS CONTORTUS USING COMPARATIVE PCR-RFLPMETHODS. IRANIAN JOURNAL OF PARASITOLOGY, 6(2), 45-53. SID. https://sid.ir/paper/303934/en

Vancouver: Copy

NABAVI R., SHAYAN P., SHOKRANI H.R., ESLAMI A., BOKAIE S.. EVALUATION OF BENZIMIDAZOLE RESISTANCE IN HAEMONCHUS CONTORTUS USING COMPARATIVE PCR-RFLPMETHODS. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2011;6(2):45-53. Available from: https://sid.ir/paper/303934/en

IEEE: Copy

R. NABAVI, P. SHAYAN, H.R. SHOKRANI, A. ESLAMI, and S. BOKAIE, “EVALUATION OF BENZIMIDAZOLE RESISTANCE IN HAEMONCHUS CONTORTUS USING COMPARATIVE PCR-RFLPMETHODS,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 6, no. 2, pp. 45–53, 2011, [Online]. Available: https://sid.ir/paper/303934/en

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