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Information Journal Paper

Title

MICROSCOPIC AND MOLECULAR DETECTION OF THEILERIA (BABESIA) EQUI INFECTION IN EQUIDS OF KURDISTAN PROVINCE, IRAN

Pages

  86-90

Keywords

PCR 

Abstract

 Background: Equine piroplasmosis (EP) is the cause of persistent tick-borne infection with no symptoms, but the most important problem of EP is due to the persistent carrier state. Carrier animals to Babesia (THEILERIA) equi (Laveran 1901) and B. caballi (Nuttall, 1910) infestation could be identified by extremely sensitive PCR-based method. The purpose of this study was to identify the causative agents of equine piroplasmosis based on molecular and microscopic assays in equids from Kurdistan Province, IRAN.Methods: Thirty one horse and mule blood samples were used with history of living in Kurdistan Province of IRAN. The blood specimens were utilized for T. equi and B. caballi DNA identification by PCR and Giemsa stained smears for micro-scopic observation.Results: The results clearly showed the presence of B. (THEILERIA) equi DNA in 30 of 31 blood samples (96.77%), but the microscopic examination revealed the 3 of 31 positive Babesia like organisms in the red blood cells (9.67%).Conclusion: The obtained results demonstrated the presence of hidden B. (THEILERIA) equi infection in horses with previous habitance in Kurdistan Province of IRAN. The carrier animals became a main source of infection and can transmit the disease. Therefore, hidden infection might be considered as a health threatening and limiting factor in animals used in therapeutic antisera research and production centers.

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    APA: Copy

    HABIBI, GHOLAMREZA, ESMAEILNIA, KASRA, HABLOLVARID, MOHAMMAD HASAN, AFSHARI, ASGHAR, ZAMEN, MOHSEN, & BOZORGI, SOGHRA. (2016). MICROSCOPIC AND MOLECULAR DETECTION OF THEILERIA (BABESIA) EQUI INFECTION IN EQUIDS OF KURDISTAN PROVINCE, IRAN. IRANIAN JOURNAL OF PARASITOLOGY, 11(1), 86-90. SID. https://sid.ir/paper/304082/en

    Vancouver: Copy

    HABIBI GHOLAMREZA, ESMAEILNIA KASRA, HABLOLVARID MOHAMMAD HASAN, AFSHARI ASGHAR, ZAMEN MOHSEN, BOZORGI SOGHRA. MICROSCOPIC AND MOLECULAR DETECTION OF THEILERIA (BABESIA) EQUI INFECTION IN EQUIDS OF KURDISTAN PROVINCE, IRAN. IRANIAN JOURNAL OF PARASITOLOGY[Internet]. 2016;11(1):86-90. Available from: https://sid.ir/paper/304082/en

    IEEE: Copy

    GHOLAMREZA HABIBI, KASRA ESMAEILNIA, MOHAMMAD HASAN HABLOLVARID, ASGHAR AFSHARI, MOHSEN ZAMEN, and SOGHRA BOZORGI, “MICROSCOPIC AND MOLECULAR DETECTION OF THEILERIA (BABESIA) EQUI INFECTION IN EQUIDS OF KURDISTAN PROVINCE, IRAN,” IRANIAN JOURNAL OF PARASITOLOGY, vol. 11, no. 1, pp. 86–90, 2016, [Online]. Available: https://sid.ir/paper/304082/en

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