AFFINITY CHROMATOGRAPHY PURIFICATION OF VEROTOXINL FROM VEROTOXIGENIC STRAINS OF ESCHERICHIA COLI (VTEC)

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SATARI MORTEZA; ZAHIR MOHAMMAD HASSAN; ALAMEH A.A.; HOSEINZADEH GAKBAN H.

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Journal: DANESHVAR MEDICINE Year:2004 | Volume:11 | Issue:50 Page(s): 31-36

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Title

AFFINITY CHROMATOGRAPHY PURIFICATION OF VEROTOXINL FROM VEROTOXIGENIC STRAINS OF ESCHERICHIA COLI (VTEC)

Author(s)

SATARI MORTEZA | ZAHIR MOHAMMAD HASSAN | ALAMEH A.A. | HOSEINZADEH GAKBAN H. | Issue Writer Certificate

Keywords

VEROTOXINLQ3

GLYCOLIPIDQ4

VEROTOXIGENIC E.COLIQ4

Abstract

Vero toxins are a group of closely related subunit toxins, that have significant effects in pathogenesis of verotoxigenic Escherichia coli. Recent evidences suggest that vero toxinl induce cytotoxic effects on some tumor cell lines. So isolation of verotoxigenic Escherichia coli strains and toxin purification are very important subjects. Inspite of different genetically desigened methods in detection of vera toxigenic Escherichia coli strains ,serological detection with polyclonal anti-verotoxinantibodies is a very rapid and important method.Ourpurpose ispurification ofVEROTOXINL from screened diarrheagenic strains, to preparation of serologicalldt in the future.Toxin production in five diarrehagenicstrains was screened by Vero toxin-producing Escherichia coli-Reverse passive latex agglutination test (VTEC-RPLA kit, Oxoid, Co, Ltd) and then purified by affinity chromathgraphy of receptor GLYCOLIPID (PI blood group antigen).Screened strain produced large amounts of toxin that have been comparable with standard toxin. Electrophoresis of toxin indicate an intense and sharp band in rage of 70 kilodalton. Intraperitoneal injection in Balb/c have several effects such as hind-legparalysis, cachexiasand diarrheae.In addition in exposed mouse and Hela and Vera cell lines teratment,we observed severe cytotoxic effects.

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APA: Copy

SATARI, MORTEZA, ZAHIR, MOHAMMAD HASSAN, ALAMEH, A.A., & HOSEINZADEH GAKBAN, H.. (2004). AFFINITY CHROMATOGRAPHY PURIFICATION OF VEROTOXINL FROM VEROTOXIGENIC STRAINS OF ESCHERICHIA COLI (VTEC). DANESHVAR MEDICINE, 11(50), 31-36. SID. https://sid.ir/paper/30427/en

Vancouver: Copy

SATARI MORTEZA, ZAHIR MOHAMMAD HASSAN, ALAMEH A.A., HOSEINZADEH GAKBAN H.. AFFINITY CHROMATOGRAPHY PURIFICATION OF VEROTOXINL FROM VEROTOXIGENIC STRAINS OF ESCHERICHIA COLI (VTEC). DANESHVAR MEDICINE[Internet]. 2004;11(50):31-36. Available from: https://sid.ir/paper/30427/en

IEEE: Copy

MORTEZA SATARI, MOHAMMAD HASSAN ZAHIR, A.A. ALAMEH, and H. HOSEINZADEH GAKBAN, “AFFINITY CHROMATOGRAPHY PURIFICATION OF VEROTOXINL FROM VEROTOXIGENIC STRAINS OF ESCHERICHIA COLI (VTEC),” DANESHVAR MEDICINE, vol. 11, no. 50, pp. 31–36, 2004, [Online]. Available: https://sid.ir/paper/30427/en

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