SCHWANN CELL CULTURE WITHOUT INHIBITOR FACTORS

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FIROUZI M.; SABOUNI F.

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Journal Paper

Paper Information

Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2001 | Volume:8 | Issue:30 Page(s): 71-93

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Information Journal Paper

Title

SCHWANN CELL CULTURE WITHOUT INHIBITOR FACTORS

Author(s)

FIROUZI M. | SABOUNI F. | Issue Writer Certificate

Keywords

SCHWANN CELLSQ4

CELL CULTUREQ2

NERVOUS SYSTEMQ3

SCIATIC NERVEQ3

Abstract

SCHWANN CELLS support neuronal function in the intact nerve by supplying the myelin sheath around axons. SCHWANN CELLS are likely to regulate the ionic environment of axons, provide neurotrophic support and regulate the periaxonal space. SCHWANN CELLS culture facilitates analysis of both normal schwann cell function and associated diseases, while, it is the primmy effect is on schwann cell function. Dsewes in this category include neurofibromatosis, associated with loss of schwann cell growth control, and so on. In vivo, SCHWANN CELLS survive in axonal degeneration following a cut or crush injury. SCHWANN CELLS can be cultured from any peripheral nerve. SCHWANN CELLS were isolated from SCIATIC NERVEs of day 14 (E14)chick embryo and postnatal rat (1-2 days after birth) SCIATIC NERVEs and donal root ganglia (DRG) of day 8 (E8) chick embryo.This technique differs from otheres who described the use of whole ganglion or SCIATIC NERVE rather than dissociated preparation. The SCIATIC NERVEs were chopped into pieces of 3 mm lenghth and transferred into a 35 mm Petri dish immersed in DMEM medium. The cultures were not over grown by fib rob lasts. After 48 hr incubation the primary cultures were transferred into another dish containing fresh medium After further 48 hr incubation the step was repeated again. SCIATIC NERVEs were trans fered in to gel collagen covered plates containing DMEM and 10% FCS. After four to five weeks in cubation greatly enriched schwann CELL CULTUREs were achieved.

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APA: Copy

FIROUZI, M., & SABOUNI, F.. (2001). SCHWANN CELL CULTURE WITHOUT INHIBITOR FACTORS. DANESHVAR MEDICINE, 8(30), 71-93. SID. https://sid.ir/paper/30487/en

Vancouver: Copy

FIROUZI M., SABOUNI F.. SCHWANN CELL CULTURE WITHOUT INHIBITOR FACTORS. DANESHVAR MEDICINE[Internet]. 2001;8(30):71-93. Available from: https://sid.ir/paper/30487/en

IEEE: Copy

M. FIROUZI, and F. SABOUNI, “SCHWANN CELL CULTURE WITHOUT INHIBITOR FACTORS,” DANESHVAR MEDICINE, vol. 8, no. 30, pp. 71–93, 2001, [Online]. Available: https://sid.ir/paper/30487/en

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