INHIBITION OF GENE EXPRESSION BY RNA INTERFERENCE (RNAI) IN P19 EMBRYONAL CARCINOMA CELL LINE

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ESMAEILI FARIBA

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2009 | Volume:16 | Issue:78 Page(s): 5-10

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Title

INHIBITION OF GENE EXPRESSION BY RNA INTERFERENCE (RNAI) IN P19 EMBRYONAL CARCINOMA CELL LINE

Author(s)

ESMAEILI FARIBA | Issue Writer Certificate

Keywords

GENE SILENCINGQ3

RNA INTERFERENCE (RNAI)Q3

SMALL INTERFERING RNAS (SIRNA)Q3

P19 EMBRYONAL CARCINOMA (EC) STEM CELLQ3

Abstract

Introduction: RNA interference (RNAi) is a phenomenon of GENE SILENCING that uses double-stranded RNA (dsRNA), specifically inhibits gene expression by degrading mRNA efficiently. The mediators of degradation are 21- to 23-nt small interfering RNAs (siRNA). The use of siRNAs as inhibitors of gene expression has been shown to be an effective way of studying gene function in mammalian cells.Aim: To investigate the efficiency of siRNAs to eGFP GENE SILENCING in P19 embryonal carcinoma (EC) stem cell.Materials & Methods: Here, we used a vector-based siRNA expression system, pSUPER that can induce RNAi in mammalian cells (P19 line of murine embryonal carcinoma stem cell). The vector containing a small hairpin RNA (shRNA) to target exogenous reporter gene, enhanced green fluorescent protein (eGFP). The expression of eGFP in the cells was detected by using fluorescent microscopy and flow cytometry.Results: Using eGFP as a reporter system, we show here that a short hairpin RNA (shRNA) expression vector can specifically inhibit gene expression. Ttransfection of the plasmid into P19 cells significantly decreased the number of eGFP-expressing cells and overall eGFP fluorescence. The RNA interfering effect was successfully observed in both transient and stable transfected cells. Conclusion: The results indicate that use of hairpin siRNA expressing vectors for RNAi is a promising method to inhibition of gene expression in mammalian cells.

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APA: Copy

ESMAEILI, FARIBA. (2009). INHIBITION OF GENE EXPRESSION BY RNA INTERFERENCE (RNAI) IN P19 EMBRYONAL CARCINOMA CELL LINE. DANESHVAR MEDICINE, 16(78), 5-10. SID. https://sid.ir/paper/30531/en

Vancouver: Copy

ESMAEILI FARIBA. INHIBITION OF GENE EXPRESSION BY RNA INTERFERENCE (RNAI) IN P19 EMBRYONAL CARCINOMA CELL LINE. DANESHVAR MEDICINE[Internet]. 2009;16(78):5-10. Available from: https://sid.ir/paper/30531/en

IEEE: Copy

FARIBA ESMAEILI, “INHIBITION OF GENE EXPRESSION BY RNA INTERFERENCE (RNAI) IN P19 EMBRYONAL CARCINOMA CELL LINE,” DANESHVAR MEDICINE, vol. 16, no. 78, pp. 5–10, 2009, [Online]. Available: https://sid.ir/paper/30531/en

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