EVALUATION OF SERIAL THAWING-REFREEZING ON HUMAN SPERMATOZOA RESISTANCE USING CRYOVIALS AND STRAWS

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GHASEMIAN FATEMEH; FARAJI ROYA; MOHAMMADI SARDOO MOHADDESE; BAHADORI MOHAMMAD HADI

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Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2012 | Volume:6 | Issue:3 Page(s): 157-164

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Title

EVALUATION OF SERIAL THAWING-REFREEZING ON HUMAN SPERMATOZOA RESISTANCE USING CRYOVIALS AND STRAWS

Author(s)

GHASEMIAN FATEMEH | FARAJI ROYA | MOHAMMADI SARDOO MOHADDESE | BAHADORI MOHAMMAD HADI | Issue Writer Certificate

Keywords

SEMEN

OLIGOZOOSPERMIA

FREEZING - THAWING

Abstract

Background: We designed this study to detect the cryoinjury rate on human sperm after serial freezing and thawing, taking into consideration the effects of using cryovials and straws.Materials and Methods: In this experimental study, SEMEN specimens obtained from 15 subjects were divided into normozoospermic and oligozoospermic groups. Each of the normozoospermic and oligozoo spermic SEMEN specimens were additionally divided into two groups: i. washed and ii. unwashed. Specimens were repeatedly freeze-thawed by using cryovials and straws with the fast liquid nitrogen vapor method, until no motile sperm remained. Sperm motility, recovery, and morphology rate were then determined after thawing, and compared between the groups while taking into consideration the effects of using cryovials and straws.Results: Motile spermatozoa were observed in all normozoospermic samples up to thaw 6 with both cryovials and straws while in oligozoospermic specimens up to thaw 4 (straw) and thaw 3 (cryovial) in the freeze-thawing cycle. Normozoospermic sample analysis showed no significant difference in morphology rate. There was a significant increase in motility and recovery percentages for washed samples, which was observed with straws in compared to the unwashed groups. Oligozoospermic sample analysis indicated a significant increase in motility, recovery (p<0.01), and morphology (p<0.001) rates in washed specimens compared to unwashed specimens using straws. The importance of washing sperm was obvious for oligozoospermic specimens.Conclusion: Normozoospermic sperm resisted freezing longer than oligozoospermic sperm. Use of straws and cryovials made significant differences in motility, recovery, and morphology of sperm in each thaw. This difference was slightly higher for oligozoospermic specimens. Results indicated that the percentage of motility was higher for washed normozoospermic specimens in each thaw when straws were used, whereas the percentage of motility, recovery, and morphology were promoted after frozen oligozoospermic specimens were washed using straws.

Cites

METHODS OF CRYOPRESERVING SPERM IN INFERTILE MEN WITH OLIGOSPERMIA AND CRYPTOSPERMIA PATTERNS

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APA: Copy

GHASEMIAN, FATEMEH, FARAJI, ROYA, MOHAMMADI SARDOO, MOHADDESE, & BAHADORI, MOHAMMAD HADI. (2012). EVALUATION OF SERIAL THAWING-REFREEZING ON HUMAN SPERMATOZOA RESISTANCE USING CRYOVIALS AND STRAWS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 6(3), 157-164. SID. https://sid.ir/paper/305374/en

Vancouver: Copy

GHASEMIAN FATEMEH, FARAJI ROYA, MOHAMMADI SARDOO MOHADDESE, BAHADORI MOHAMMAD HADI. EVALUATION OF SERIAL THAWING-REFREEZING ON HUMAN SPERMATOZOA RESISTANCE USING CRYOVIALS AND STRAWS. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2012;6(3):157-164. Available from: https://sid.ir/paper/305374/en

IEEE: Copy

FATEMEH GHASEMIAN, ROYA FARAJI, MOHADDESE MOHAMMADI SARDOO, and MOHAMMAD HADI BAHADORI, “EVALUATION OF SERIAL THAWING-REFREEZING ON HUMAN SPERMATOZOA RESISTANCE USING CRYOVIALS AND STRAWS,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 6, no. 3, pp. 157–164, 2012, [Online]. Available: https://sid.ir/paper/305374/en

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