THE EFFECTS OF OLIVE LEAF EXTRACT ON THE TESTIS, SPERM QUALITY ANDTESTICULAR GERM CELL APOPTOSIS IN MALE RATS EXPOSED TO BUSULFAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GANJALIKHAN HAKEMI SEPIDEH; SHARIFIFAR FARIBA; HAGHPANAH TAHEREH; BABAEE ABDOLREZA; EFTEKHAR VAGHEFI SEYED HASSAN

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: International Journal of Fertility & Sterility Year:2019 | Volume:13 | Issue:1 Page(s): 57-65

Download Full-Text

View:

298

Download:

412

Cites:

Information Journal Paper

Title

THE EFFECTS OF OLIVE LEAF EXTRACT ON THE TESTIS, SPERM QUALITY ANDTESTICULAR GERM CELL APOPTOSIS IN MALE RATS EXPOSED TO BUSULFAN

Author(s)

Keywords

APOPTOSIS

BUSULFAN

OLIVE EXTRACT

SPERMATOGENESIS

TESTICULAR GERM CELL

Abstract

Background: BUSULFAN (BU) has a destructive effect on the male reproductive system. The goal of this study was toassess the effects of olive leaf extract (OLE) as a source of antioxidants and phenolic compounds, on BU-induceddamages in rat testes. Materials and Methods: In this experimental study, 40 male Wistar rats were randomly divided into 5 groups. Thecontrol group (CTL) received a single intraperitoneal (i. p. ) injection of dimethyl sulfoxide (DMSO), followed byoral administration of distilled water for 5 weeks. In BU group, BU (10 mg/kg) was administrated i. p. once. In cotreatmentgroups, first, received BU (10 mg/kg, a single i. p. injection) then, OLE was administrated orally at differentdoses of 250 mg/kg (BU+OLE 250), 500 mg/kg (BU+OLE 500) and 750 mg/kg (BU+OLE 750), for 5 weeks. Next, blood and sperm samples were collected. The left testis was removed to investigate testicular parameters and APOPTOSISby using H&E and TUNEL staining, respectively. All data were analyzed by SPSS software and a P<0. 05 wasconsidered significant. Results: There was a significant decline in sperm viability (P=0. 017), number of primary spermatocyte (PS) (P=0. 001)and Leydig cells (P=0. 023) in the BU group versus the CTL group. OLE at three doses could repair these defects versusBU group. Increases in apoptotic spermatogonia cells (SG) due to BU were significantly reduced by OLE 250and 500 mg/kg (P<0. 01). A reduction in germinal epithelium height and an increase in apoptotic SG were observed inBU+OLE 750 group vs. other groups (P<0. 01) and alkaline phosphatase (ALP) was at the highest level, also Aspartateaminotransferase (AST) increased markedly vs. CTL (P=0. 024). Conclusion: Oral administration of OLE at the doses of 250 and 500 mg/kg could be helpful in ameliorating BUinducedtoxicity in rat testes, while OLE 750 mg/kg not only did not cause positive effects, but also could exacerbatethe harmful effects.

Cites

Crocin Reduces Oxidative Stress Produced by Busulfan Administration on Fertility of Male Rats

References

No record.

Cite

APA: Copy

GANJALIKHAN HAKEMI, SEPIDEH, SHARIFIFAR, FARIBA, HAGHPANAH, TAHEREH, BABAEE, ABDOLREZA, & EFTEKHAR VAGHEFI, SEYED HASSAN. (2019). THE EFFECTS OF OLIVE LEAF EXTRACT ON THE TESTIS, SPERM QUALITY ANDTESTICULAR GERM CELL APOPTOSIS IN MALE RATS EXPOSED TO BUSULFAN. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, 13(1), 57-65. SID. https://sid.ir/paper/306034/en

Vancouver: Copy

GANJALIKHAN HAKEMI SEPIDEH, SHARIFIFAR FARIBA, HAGHPANAH TAHEREH, BABAEE ABDOLREZA, EFTEKHAR VAGHEFI SEYED HASSAN. THE EFFECTS OF OLIVE LEAF EXTRACT ON THE TESTIS, SPERM QUALITY ANDTESTICULAR GERM CELL APOPTOSIS IN MALE RATS EXPOSED TO BUSULFAN. INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY[Internet]. 2019;13(1):57-65. Available from: https://sid.ir/paper/306034/en

IEEE: Copy

SEPIDEH GANJALIKHAN HAKEMI, FARIBA SHARIFIFAR, TAHEREH HAGHPANAH, ABDOLREZA BABAEE, and SEYED HASSAN EFTEKHAR VAGHEFI, “THE EFFECTS OF OLIVE LEAF EXTRACT ON THE TESTIS, SPERM QUALITY ANDTESTICULAR GERM CELL APOPTOSIS IN MALE RATS EXPOSED TO BUSULFAN,” INTERNATIONAL JOURNAL OF FERTILITY AND STERILITY, vol. 13, no. 1, pp. 57–65, 2019, [Online]. Available: https://sid.ir/paper/306034/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops