Expression of human FSH in Chinese hamster ovarian cells and its activity in a rat model

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

Mousivand Azam; Timurian Shahram; Shahabi Amirhassan; RASOOLI IRAJ

Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DANESHVAR MEDICINE Year:2019 | Volume:26 | Issue:139 Page(s): 1-8

Download Full-Text

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Persian Verion

View:

530

Download:

Cites:

Information Journal Paper

Title

Expression of human FSH in Chinese hamster ovarian cells and its activity in a rat model

Author(s)

Mousivand Azam | Timurian Shahram | Shahabi Amirhassan | RASOOLI IRAJ | Issue Writer Certificate

Keywords

E.coliQ2

Follicle stimulating hormone (FSH)Q2

ExpressionQ3

Chinese hamster ovary cell line (CHO)Q2

Abstract

Background and Objective: FSH is a heterodimeric glycoprotein that performs an important role in the regulation of reproductive processes. Over the past decades, injectable gonadotrophins have played a leading role in the treatment of infertility. The production of FSH in the country is one of the main goals around this drug. The aim of this study was to produce a semi-industrial recombinant FSH. Materials and Methods: In this research study, the FSH gene contained within the pVITRO2-neo-mcs vector was transfected into the cell line of CHO after transformation in DH5α and plasmid purification. The amount of FSH in cell supernatant was measured by ELISA. The FSH protein was purified by HPLC. The presence of FSH protein after purification was confirmed by SDS-PAGE and Western blotting methods. Finally, the clinical effects of purified FSH on the ovary in the rat model were investigated. Results: The Expression of the protein was determined by ELISA test at 450 nm that was 43. 538 mIU/ml. In the HPLC purification step, the FSH peak was observed at a time interval of about 7. 9 minutes, and the purification efficiency was 70%. The existence of a 45 KDa band on the pvdf membrane was confirmed after western blotting of FSH. Increasing the weight of the ovary after the injection has confirmed the functionality of the protein. Conclusion: At present, the production of recombinant hormone is one of the basic needs of every country. In order to arrive at this aim, a long-term planning is required. We are trying to pave the way for producing human FSH hormone as an effective recombinant drug in the treatment of infertility.

Cites

No record.

References

No record.

Cite

APA: Copy

Mousivand, Azam, Timurian, Shahram, Shahabi, Amirhassan, & RASOOLI, IRAJ. (2019). Expression of human FSH in Chinese hamster ovarian cells and its activity in a rat model. DANESHVAR MEDICINE, 26(139 ), 1-8. SID. https://sid.ir/paper/30609/en

Vancouver: Copy

Mousivand Azam, Timurian Shahram, Shahabi Amirhassan, RASOOLI IRAJ. Expression of human FSH in Chinese hamster ovarian cells and its activity in a rat model. DANESHVAR MEDICINE[Internet]. 2019;26(139 ):1-8. Available from: https://sid.ir/paper/30609/en

IEEE: Copy

Azam Mousivand, Shahram Timurian, Amirhassan Shahabi, and IRAJ RASOOLI, “Expression of human FSH in Chinese hamster ovarian cells and its activity in a rat model,” DANESHVAR MEDICINE, vol. 26, no. 139 , pp. 1–8, 2019, [Online]. Available: https://sid.ir/paper/30609/en

Related Journal Papers