MOLECULAR EPIDEMIOLOGY OF DIFFERENT HEPATITIS C GENOTYPES IN SERUM AND PERIPHERAL BLOOD MONONUCLEAR CELLS IN JAHROM CITY OF IRAN

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

ASHRAFI HAFEZ ASGHAR; BAHARLOU RASOUL; MOUSAVI NASAB SEYED DAWOOD; AHMADI VASMEHJANI ABBAS; SHAYESTEHPOUR MOHAMMAD; JOHARINIA NEGAR; AHMADI NAYEB ALI

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Hepatitis Monthly Year:2014 | Volume:14 | Issue:5 Page(s): 0-0

Download Full-Text

View:

375

Download:

129

Cites:

Information Journal Paper

Title

MOLECULAR EPIDEMIOLOGY OF DIFFERENT HEPATITIS C GENOTYPES IN SERUM AND PERIPHERAL BLOOD MONONUCLEAR CELLS IN JAHROM CITY OF IRAN

Author(s)

Keywords

HEPATITIS C VIRUS

GENOTYPE

POLYMERASE CHAIN REACTION

Abstract

Background: The HEPATITIS C VIRUS (HCV) is considered essentially hepatotropic, yet the virus compartments have also been found in important extra hepatic sites. Detection of HCV RNA in extra hepatic reservoirs such as peripheral blood mononuclear cells (PBMCs) is important for determining disease progression and treatment effectiveness.Objectives: The present study aimed to determine different HCV GENOTYPEs in patients' plasma and PBMC specimens, in Jahrom city of Iran.Patients and Methods: Blood samples of 137 patients with established HCV were collected at the Honari clinic. These patients were anti-HCV and plasma HCV RNA positive. After plasma RNA extraction and obtaining a pellet of approximately 3-5×106 PBMCs, Real-time PCR was performed, using specific-genotype primers. Finally, data analysis was done by the Statistical Package for Social Sciences (SPSS) software.Results: Subtype 3 was the most common GENOTYPE in plasma (57.7%) and PBMCs (51.1%). Subtype 1a was detected in 36.5% and 30.7% of plasma samples and PBMCs, respectively whereas subtype 4 was not detected in any of the cases. There was a GENOTYPE difference between plasma and PBMCs of 12.4% of patients. In four patients no GENOTYPE was detected in their plasma but GENOTYPE 3 was detected in the PBMCs.Conclusions: It is suggested that determination of the target GENOTYPE by plasma subtyping for choosing the proper antiviral therapy is essential but may result in therapy failure. HCV genotyping in PBMC samples, along with plasma specimens, might be more beneficial. Therefore determining the HCV GENOTYPE in PBMCs, before beginning the therapy is useful due to the possibility of occult infection detection.

Cites

PREVALENCE OF HEPATITIS C VIRUS GENOTYPES AMONG PATIENTS IN COUNTRIES OF THE EASTERN MEDITERRANEAN REGIONAL OFFICE OF WHO (EMRO): A SYSTEMATIC REVIEW AND META-ANALYSIS

References

No record.

Cite

APA: Copy

ASHRAFI HAFEZ, ASGHAR, BAHARLOU, RASOUL, MOUSAVI NASAB, SEYED DAWOOD, AHMADI VASMEHJANI, ABBAS, SHAYESTEHPOUR, MOHAMMAD, JOHARINIA, NEGAR, & AHMADI, NAYEB ALI. (2014). MOLECULAR EPIDEMIOLOGY OF DIFFERENT HEPATITIS C GENOTYPES IN SERUM AND PERIPHERAL BLOOD MONONUCLEAR CELLS IN JAHROM CITY OF IRAN. HEPATITIS MONTHLY, 14(5), 0-0. SID. https://sid.ir/paper/306644/en

Vancouver: Copy

ASHRAFI HAFEZ ASGHAR, BAHARLOU RASOUL, MOUSAVI NASAB SEYED DAWOOD, AHMADI VASMEHJANI ABBAS, SHAYESTEHPOUR MOHAMMAD, JOHARINIA NEGAR, AHMADI NAYEB ALI. MOLECULAR EPIDEMIOLOGY OF DIFFERENT HEPATITIS C GENOTYPES IN SERUM AND PERIPHERAL BLOOD MONONUCLEAR CELLS IN JAHROM CITY OF IRAN. HEPATITIS MONTHLY[Internet]. 2014;14(5):0-0. Available from: https://sid.ir/paper/306644/en

IEEE: Copy

ASGHAR ASHRAFI HAFEZ, RASOUL BAHARLOU, SEYED DAWOOD MOUSAVI NASAB, ABBAS AHMADI VASMEHJANI, MOHAMMAD SHAYESTEHPOUR, NEGAR JOHARINIA, and NAYEB ALI AHMADI, “MOLECULAR EPIDEMIOLOGY OF DIFFERENT HEPATITIS C GENOTYPES IN SERUM AND PERIPHERAL BLOOD MONONUCLEAR CELLS IN JAHROM CITY OF IRAN,” HEPATITIS MONTHLY, vol. 14, no. 5, pp. 0–0, 2014, [Online]. Available: https://sid.ir/paper/306644/en

Related Journal Papers

No record.

Related Seminar Papers

No record.

Related Plans

No record.

Recommended Workshops