EFFECT OF VITRIFICATION ON THE SURVIVAL AND DEVELOPMENT OF MII MOUSE OOCYTE BY TWO THAWING PROCEDURES

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SALEHNIA M.; REZAZADEH M.; TAKI M.

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Journal: DANESHVAR MEDICINE Year:2000 | Volume:7 | Issue:27 Page(s): 81-86

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Title

EFFECT OF VITRIFICATION ON THE SURVIVAL AND DEVELOPMENT OF MII MOUSE OOCYTE BY TWO THAWING PROCEDURES

Author(s)

SALEHNIA M. | REZAZADEH M. | TAKI M. | Issue Writer Certificate

Keywords

VITRIFICATIONQ3

THAWING PROCEDUREQ4

ETHYLENE GLYCOLQ3

MII OOCYTEQ4

Abstract

We investigated the effect of THAWING PROCEDURE by two or three steps on the survival, fertilization and developmental rate of vitrified MII mouse oocyte. Female NMRI mice (6-10 weeks old) were superovulated using IP injection of 10 IU human Monoposal Gonadotropin (hMG) and 10 IU human Chorionic Gonadotropin (hCG). The ovulated oocytes were then collected from the ampullary portion of the oviducts at 12-14 hours after hCG injection. The cumulus cells mass were apart by 0.1% hyaluronidase. The oocytes were vitrified by a solution of PBI contained 30 % (W/V) ficoll 70, 0.5 M sucrose, 10.7% (W/V) acetamid and 10% (V/V) ETHYLENE GLYCOL and stored in liquid nitrogen. The frozen oocytes were thawed using sucrose solutions by two and three step manner (the group 1: with 0.5 M surose and PBI, the group 2 with 1 and 0.5 M sucrose and PBI). Then the oocytes were inseminated with epididymal sperm, cultured in T6 medium. Control non frozen oocytes were also inseminated. The survival rate of vitrified oocytes after thawing in the group 1 was 79% and in the group 2 was 76.1 %. The fertilization rates of the group 1, 2 and control were 40.8%, 45.4% & 48% respectively. The developmental rates of the group 1, 2 and control were 95%, 95% and 96% respectively. There was no significant difference between three groups. The THAWING PROCEDURE by two or three steps had no effect on the survival and development of oocyte. This VITRIFICATION method is a simple and useful procedure for cryopreservation of oocytes.

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References

انجماد شیشه ای تخمک متافاز II موش با استفاده از ضد یخ اتیلن گلیکول

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APA: Copy

SALEHNIA, M., REZAZADEH, M., & TAKI, M.. (2000). EFFECT OF VITRIFICATION ON THE SURVIVAL AND DEVELOPMENT OF MII MOUSE OOCYTE BY TWO THAWING PROCEDURES. DANESHVAR MEDICINE, 7(27), 81-86. SID. https://sid.ir/paper/30790/en

Vancouver: Copy

SALEHNIA M., REZAZADEH M., TAKI M.. EFFECT OF VITRIFICATION ON THE SURVIVAL AND DEVELOPMENT OF MII MOUSE OOCYTE BY TWO THAWING PROCEDURES. DANESHVAR MEDICINE[Internet]. 2000;7(27):81-86. Available from: https://sid.ir/paper/30790/en

IEEE: Copy

M. SALEHNIA, M. REZAZADEH, and M. TAKI, “EFFECT OF VITRIFICATION ON THE SURVIVAL AND DEVELOPMENT OF MII MOUSE OOCYTE BY TWO THAWING PROCEDURES,” DANESHVAR MEDICINE, vol. 7, no. 27, pp. 81–86, 2000, [Online]. Available: https://sid.ir/paper/30790/en

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