STUDY OF RELATIONSHIP BETWEEN RADIOSENSITIVITY, CHROMOSOME SIZE & MORPHOLOGY IN RADIOSENSITIVE MUTANTS &THEIR NORMAL PARENTAL CELL LINES

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Journal: Daneshvar Medicine: Basic and Clinical Research Journal Year:2000 | Volume:7 | Issue:27 Page(s): 71-80

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Title

STUDY OF RELATIONSHIP BETWEEN RADIOSENSITIVITY, CHROMOSOME SIZE & MORPHOLOGY IN RADIOSENSITIVE MUTANTS &THEIR NORMAL PARENTAL CELL LINES

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Keywords

RADIOSENSITIVE MUTANT CELL LINESQ4

RADIOSENSITIVITYQ4

CHROMOSOME SIZE AND MORPHOLOGYQ4

Abstract

Xrs-5 and xrs-7 are radiation - sensitive mutants of the Chinese hamster ovary (CHO) cell lines. These cells show a defect in rejoining of DNA double strand breaks and alterations in chromosome structure and morphology. Irs1 is also another Chinese hamster mutant derived from V79 cells with a high sensitivity to radiation and chemicals. The relationship between radiation sensitivity and metaphase CHROMOSOME SIZE AND MORPHOLOGY was examined in these mutants and their parental wild type cells. Cells were grown in MEM medium and after colcemid treatment, metaphase was prepared using standard methods. Slides were air dried and left on hot surface over night at 54°C and stained in Dapi. Metaphases were analysed using a computerized system, Smartcapture VP, and measurements were performed using IP Lab software. Results indicate a significant difference between the length, thickness and ratio of thickness to length of chromosomes xrs-5 to CHO-K1 andxrs-7 cells (P<0.001). Xrs-5 Chromosomes were seen to be over- condensed and shorter. In contrast, length of irs1 cells was seen to be longer than its parental lines, V79 and V79-4. This is also found to be significantly different from each other (P <0. 001). These results suggest that there is a fundamental difference between mutant and parental cell~ in either the organization of the loops of DNA attached to the nuclear matrix or in the nature of the proteins that attach the DNA to the matrix. However, Chromosome size and over - condensed morphology might not play a role in RADIOSENSITIVITY of cells, but might be due to alterations in the phosphorylation of chromatin proteins.

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APA: Copy

. (2000). STUDY OF RELATIONSHIP BETWEEN RADIOSENSITIVITY, CHROMOSOME SIZE & MORPHOLOGY IN RADIOSENSITIVE MUTANTS &THEIR NORMAL PARENTAL CELL LINES. DANESHVAR MEDICINE, 7(27), 71-80. SID. https://sid.ir/paper/30794/en

Vancouver: Copy

. STUDY OF RELATIONSHIP BETWEEN RADIOSENSITIVITY, CHROMOSOME SIZE & MORPHOLOGY IN RADIOSENSITIVE MUTANTS &THEIR NORMAL PARENTAL CELL LINES. DANESHVAR MEDICINE[Internet]. 2000;7(27):71-80. Available from: https://sid.ir/paper/30794/en

IEEE: Copy

, “STUDY OF RELATIONSHIP BETWEEN RADIOSENSITIVITY, CHROMOSOME SIZE & MORPHOLOGY IN RADIOSENSITIVE MUTANTS &THEIR NORMAL PARENTAL CELL LINES,” DANESHVAR MEDICINE, vol. 7, no. 27, pp. 71–80, 2000, [Online]. Available: https://sid.ir/paper/30794/en

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