A STUDY ON THE EFFECTS OF PROPAMIDINE ISETHIONATE AND POLYMYXIN B ON ACANTHAMOEBA ISOLATED FROM PATIENTS INFECTED WITH ACANTHAMOEBA KERATITIS IN HELA CELL CULTURE

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DELAVARI MAHDI; DALIMIASL A.A.H.; GHAFARIFAR F.; KHOUSHZABAN F.; MAROUFI Y.

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Journal: DANESHVAR MEDICINE Year:2007 | Volume:14 | Issue:70 Page(s): 27-32

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Title

A STUDY ON THE EFFECTS OF PROPAMIDINE ISETHIONATE AND POLYMYXIN B ON ACANTHAMOEBA ISOLATED FROM PATIENTS INFECTED WITH ACANTHAMOEBA KERATITIS IN HELA CELL CULTURE

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Keywords

ACANTHAMOEBAQ2

PROPAMIDINE ISETHIONATEQ3

POLYMYXIN BQ3

ACANTHAMOEBA KERATITISQ3

HELA CELL CULTUREQ4

Abstract

Background: ACANTHAMOEBA is free-living amoeba that is found in soil, water, air as well as in pharynx of human. It is the causative agent of amoebic keratitis in people who use contact lens. In the present study, the effects of PROPAMIDINE ISETHIONATE and POLYMYXIN B on ACANTHAMOEBA isolated from patients infected with ACANTHAMOEBA keratitis in HELA CELL CULTURE was investigated.Materials & Methods: To carry out the research, the isolates were cultured in NNA and axenic medium first. Then they were transferred to the wells of HELA CELL CULTURE. After 24 and 48 hours of incubation period, PROPAMIDINE ISETHIONATE or POLYMYXIN B were separately added to HELA CELL CULTURE (5 mg/mland 10 mg/ml PROPAMIDINE ISETHIONATE, 5 IU and 10 IU POLYMYXIN B) And the growth of the parasites within the drugs was measured after 24 hours.Results: The results indicated that all concentrations showed significant difference in the control group. Moreover, 10 mg/ml of PROPAMIDINE ISETHIONATE showed the highest inhibitory effect on the parasite growth. Conclusion: The effect of varied doses of above - mentioned drugs on parasite isolates was different, and increase in time duration of parasite growth led to decrease in drug's efficiency.

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APA: Copy

DELAVARI, MAHDI, DALIMIASL, A.A.H., GHAFARIFAR, F., KHOUSHZABAN, F., & MAROUFI, Y.. (2007). A STUDY ON THE EFFECTS OF PROPAMIDINE ISETHIONATE AND POLYMYXIN B ON ACANTHAMOEBA ISOLATED FROM PATIENTS INFECTED WITH ACANTHAMOEBA KERATITIS IN HELA CELL CULTURE. DANESHVAR MEDICINE, 14(70), 27-32. SID. https://sid.ir/paper/30989/en

Vancouver: Copy

DELAVARI MAHDI, DALIMIASL A.A.H., GHAFARIFAR F., KHOUSHZABAN F., MAROUFI Y.. A STUDY ON THE EFFECTS OF PROPAMIDINE ISETHIONATE AND POLYMYXIN B ON ACANTHAMOEBA ISOLATED FROM PATIENTS INFECTED WITH ACANTHAMOEBA KERATITIS IN HELA CELL CULTURE. DANESHVAR MEDICINE[Internet]. 2007;14(70):27-32. Available from: https://sid.ir/paper/30989/en

IEEE: Copy

MAHDI DELAVARI, A.A.H. DALIMIASL, F. GHAFARIFAR, F. KHOUSHZABAN, and Y. MAROUFI, “A STUDY ON THE EFFECTS OF PROPAMIDINE ISETHIONATE AND POLYMYXIN B ON ACANTHAMOEBA ISOLATED FROM PATIENTS INFECTED WITH ACANTHAMOEBA KERATITIS IN HELA CELL CULTURE,” DANESHVAR MEDICINE, vol. 14, no. 70, pp. 27–32, 2007, [Online]. Available: https://sid.ir/paper/30989/en

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