THE STUDY OF VZV GLYCOPROTEIN C (ORF 14) GENE EXPRESSION REGULATION

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FARAZ M.; SADEGHIZADEH M.

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Journal: DANESHVAR MEDICINE Year:2007 | Volume:14 | Issue:70 Page(s): 73-80

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Title

THE STUDY OF VZV GLYCOPROTEIN C (ORF 14) GENE EXPRESSION REGULATION

Author(s)

FARAZ M. | SADEGHIZADEH M. | Issue Writer Certificate

Keywords

VARICELLA ZOSTER VIRUSQ4

IN VITRO TRANSIENT EXPRESSION ASSAYQ3

GLYCOPROTEIN CQ3

GLYCOPROTEIN IQ3

GENE REGULATIONQ3

Abstract

Introduction: VZV (VARICELLA ZOSTER VIRUS) is classified as a member of the Herpesviridae family and Alphaherpesvirinae subfamily. It is the etiologic agent of two distinct clinical diseases: Chickenpox (Varicella) and shingles (Zoster). During infection, the VZV proteins are expressed in a cascade regulated manner. Immediate-early (lE) gene products are transactivators that regulate the expression of early (E) and late (L) genes. The lE genes include ORFs: 4, 61, 62 and 63. The E gene product p29 is a DNA- inding protein also known to regulate the activity of a late gene, ORF67, the product of which is GLYCOPROTEIN I (gp I). The objective of this investigation was to monitor the ability of the promoter of another late gene (ORF 14) to respond to the transregulatory activity of p29.Materials & Methods: For this purpose, primers were designed to amplify by polymerase chain reaction (PCR) which is the promoter region of ORF14 and ORF67 (as control). The PCR products were subsequently cloned in a reporter vector containing the lacZ coding sequence. After Maxi-preparation of plasmids in Transient Expression Assays, these plasmids were individually used to co transfect Huh7 and Mewo human cell lines and Vero cell line along with other plasmids which contained gene expressing transacivatory proteins.Results: Considering the system's sensitivity and positive control, it seems ORF 14 gene expression is different in three cell lines. Thus, the role of P29 is proved in Vero cell line to some extent.

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APA: Copy

FARAZ, M., & SADEGHIZADEH, M.. (2007). THE STUDY OF VZV GLYCOPROTEIN C (ORF 14) GENE EXPRESSION REGULATION. DANESHVAR MEDICINE, 14(70), 73-80. SID. https://sid.ir/paper/31001/en

Vancouver: Copy

FARAZ M., SADEGHIZADEH M.. THE STUDY OF VZV GLYCOPROTEIN C (ORF 14) GENE EXPRESSION REGULATION. DANESHVAR MEDICINE[Internet]. 2007;14(70):73-80. Available from: https://sid.ir/paper/31001/en

IEEE: Copy

M. FARAZ, and M. SADEGHIZADEH, “THE STUDY OF VZV GLYCOPROTEIN C (ORF 14) GENE EXPRESSION REGULATION,” DANESHVAR MEDICINE, vol. 14, no. 70, pp. 73–80, 2007, [Online]. Available: https://sid.ir/paper/31001/en

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