A COMPARISON OF CULTURE AND PCR METHODS WRIGHTS TEST FOR DIAGNOSIS OF BRUCELLOSIS

Q: How can I download an article?

To download an article from SID, first log in to the site, search for the article title, and click on the 'Download Article' option.

Q: How can I download an ISI article?

To download an ISI article on SID, enter the keyword or article title in the search bar, view the relevant results, click on the desired article, and select the 'Download Article' option.

Q: How can I access the SID database?

To access the SID database, visit SID.ir, create an account, and log in to access scientific resources.

Q: Is downloading articles from SID free?

Some articles on SID are available for free, while others require payment. Details are specified on the article's page.

GHOUSIAN MOGHADAM M.H.; KEYVANI AMINEH H.; ZAHRAEI SALEHI M.T.; KHAZRAEINIA P.; FALAH NADER

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: DANESHVAR MEDICINE Year:2008 | Volume:15 | Issue:74 Page(s): 51-58

Download Full-Text

Persian Verion

View:

812

Download:

Cites:

Information Journal Paper

Title

A COMPARISON OF CULTURE AND PCR METHODS WRIGHTS TEST FOR DIAGNOSIS OF BRUCELLOSIS

Author(s)

Keywords

RAW MILKQ2

BRUCELLAQ3

POLYMERASE CHAIN REACTIONQ2

CULTUREQ2

WRIGHT TESTQ3

Abstract

Background: Brucellosis is still known as a main hygienic problem in human. Pathogen is easily transmitted into human body by RAW MILK and fresh cheese. The use of rapid and sensitive diagnostic methods is essential because of the hygienic and economic importance of brucellosis.Materials & Methods: In this research, we collected 106 samples of milk and blood serum from dairy cattle that had been referred to Rey slaughterhouse. Milk samples were CULTUREd in modified Farrell's medium. In addition, according to Romero method, DNA was extracted from milk samples and PCR was done using JPR and JPF primers. WRIGHT TEST was also done on serum samples.Results: Comparison of PCR and CULTURE with WRIGHT TEST results showed that in CULTURE method sensitivity is 31.6 percent and accuracy is 48 percent but in PCR, sensitivity is 58 percent and accuracy is 65 percent.Conclusion: Results of our study showed that PCR can be attended as a suitable and rapid method to diagnose brucellosis.

Cites

No record.

References

دفع باکتری بروسلا از شیر مادران مبتلا به بروسلوز

Cite

APA: Copy

GHOUSIAN MOGHADAM, M.H., KEYVANI AMINEH, H., ZAHRAEI SALEHI, M.T., KHAZRAEINIA, P., & FALAH, NADER. (2008). A COMPARISON OF CULTURE AND PCR METHODS WRIGHTS TEST FOR DIAGNOSIS OF BRUCELLOSIS. DANESHVAR MEDICINE, 15(74), 51-58. SID. https://sid.ir/paper/31076/en

Vancouver: Copy

GHOUSIAN MOGHADAM M.H., KEYVANI AMINEH H., ZAHRAEI SALEHI M.T., KHAZRAEINIA P., FALAH NADER. A COMPARISON OF CULTURE AND PCR METHODS WRIGHTS TEST FOR DIAGNOSIS OF BRUCELLOSIS. DANESHVAR MEDICINE[Internet]. 2008;15(74):51-58. Available from: https://sid.ir/paper/31076/en

IEEE: Copy

M.H. GHOUSIAN MOGHADAM, H. KEYVANI AMINEH, M.T. ZAHRAEI SALEHI, P. KHAZRAEINIA, and NADER FALAH, “A COMPARISON OF CULTURE AND PCR METHODS WRIGHTS TEST FOR DIAGNOSIS OF BRUCELLOSIS,” DANESHVAR MEDICINE, vol. 15, no. 74, pp. 51–58, 2008, [Online]. Available: https://sid.ir/paper/31076/en

Related Journal Papers