PRODUCING A MAMMALIAN GFP EXPRESSION VECTOR CONTAINING NEOMYCIN RESISTANCE GENE

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IZADI MANIZHEH; ABIRI M.; KERAMATIPOUR M.

مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

Journal Paper

Paper Information

Journal: Avicenna Journal of Medical Biotechnology Year:2009 | Volume:1 | Issue:1 Page(s): 33-36

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Information Journal Paper

Title

PRODUCING A MAMMALIAN GFP EXPRESSION VECTOR CONTAINING NEOMYCIN RESISTANCE GENE

Author(s)

IZADI MANIZHEH | ABIRI M. | KERAMATIPOUR M. | Issue Writer Certificate

Keywords

EXPRESSION

GREEN FLOURESCENT PROTEIN

NEOMYCIN

RESISTANCE GENE

VECTOR

Abstract

The green fluorescent protein (GFP) was originally isolated from the Jellyfish Aequorea Victoria that fluoresces green when exposed to blue light. GFP protein is composed of 238 amino acids with the molecular mass of 26.9 kD. The GFP gene is frequently used in cellular and molecular biology as a reporter gene. To date, many bacterial, yeast , fungal, plants, fly and mammalian cells, including human, have been created which express GFP. Martin Chalfie, Osamu Shimomura, and Roger Tsien were awarded the 2008 noble prize in chemistry for their discovery and development of GFP. In many studies on mammalian cells, GFP gene is introduced into cells using VECTORbased systems or a recombinant virus to track the location of a target protein or to study the EXPRESSION level of the gene of interest, but in these studies there is no selection marker to normalize transfection. According to the importance of NEOMYCIN gene as a selection marker in mammalian cells, we aimed to produce a GFP EXPRESSION VECTOR that contains NEOMYCIN gene. GFP gene was separated from pEGFP-N1 VECTOR and was inserted in the backbone of pCDNA3.1/His/LacZ VECTOR that contained the NEOMYCIN gene. The resulted VECTOR contained GFP beside NEOMYCIN gene.

Cites

INVESTIGATING THE EFFECT OF RS3783605 SINGLE-NUCLEOTIDE POLYMORPHISM ON THE ACTIVITY OF VCAM-1 PROMOTER IN HUMAN UMBILICAL VEIN ENDOHELIAL CELLS

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APA: Copy

IZADI, MANIZHEH, ABIRI, M., & KERAMATIPOUR, M.. (2009). PRODUCING A MAMMALIAN GFP EXPRESSION VECTOR CONTAINING NEOMYCIN RESISTANCE GENE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), 1(1), 33-36. SID. https://sid.ir/paper/313692/en

Vancouver: Copy

IZADI MANIZHEH, ABIRI M., KERAMATIPOUR M.. PRODUCING A MAMMALIAN GFP EXPRESSION VECTOR CONTAINING NEOMYCIN RESISTANCE GENE. AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB)[Internet]. 2009;1(1):33-36. Available from: https://sid.ir/paper/313692/en

IEEE: Copy

MANIZHEH IZADI, M. ABIRI, and M. KERAMATIPOUR, “PRODUCING A MAMMALIAN GFP EXPRESSION VECTOR CONTAINING NEOMYCIN RESISTANCE GENE,” AVICENNA JOURNAL OF MEDICAL BIOTECHNOLOGY (AJMB), vol. 1, no. 1, pp. 33–36, 2009, [Online]. Available: https://sid.ir/paper/313692/en

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