Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii

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ALIBAKHSHI ABBAS; BANDEHPOUR MOJGAN; KAZEMI BAHRAM

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Journal Paper

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Journal: Iranian Journal of Microbiology Year:2017 | Volume:9 | Issue:4 Page(s): 251-256

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Information Journal Paper

Title

Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii

Author(s)

ALIBAKHSHI ABBAS | BANDEHPOUR MOJGAN | KAZEMI BAHRAM | Issue Writer Certificate

Keywords

Toxoplasma gondii

Polytopic antigen

SAG

Epitope mapping

Abstract

Background and Objectives: Polytopic antigens are recently applied for replacing crude antigens, for control of infectious agents. The surface of the Toxoplasma is covered with immunogenic antigens namely surface antigens (SAGs). These antigens possess several immunogenic epitopes, inducing immune responses. Materials and Methods: In this study, a DNA construct comprising of sequences encoding epitopes from SAG1, 2 and 3 was designed and cloned into pET28a expression vector and subsequently expressed and purified, using Ni-NTA column. Results: The SDS-PAGE and Western blotting analysis showed that polytopic genes were successfully expressed and purified. Conclusion: The surface antigenic protein of T. gondii can be applied in the future epitope-based applications.

Cites

Comparative Evaluation of Expression Vectors (pET32a and pET25b) in Expression of Polyepitopic Sequence Synthesized from Leishmania infantum Antigens

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APA: Copy

ALIBAKHSHI, ABBAS, BANDEHPOUR, MOJGAN, & KAZEMI, BAHRAM. (2017). Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii. IRANIAN JOURNAL OF MICROBIOLOGY, 9(4), 251-256. SID. https://sid.ir/paper/315519/en

Vancouver: Copy

ALIBAKHSHI ABBAS, BANDEHPOUR MOJGAN, KAZEMI BAHRAM. Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii. IRANIAN JOURNAL OF MICROBIOLOGY[Internet]. 2017;9(4):251-256. Available from: https://sid.ir/paper/315519/en

IEEE: Copy

ABBAS ALIBAKHSHI, MOJGAN BANDEHPOUR, and BAHRAM KAZEMI, “Cloning, expression and purification of a polytopic antigen comprising of surface antigens of Toxoplasma gondii,” IRANIAN JOURNAL OF MICROBIOLOGY, vol. 9, no. 4, pp. 251–256, 2017, [Online]. Available: https://sid.ir/paper/315519/en

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