A method of GC-MS analysis of serum metabolites

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SOHEILI MASOUD; AREFI OSKOUIE AFSANEH; REZAEI TAVIRANI MOSTAFA; ALIAKBARZADEH GHAZALEH; SALAMI MAHMOUD

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Journal: Archives of Advances in Biosciences Year:2018 | Volume:9 | Issue:1 Page(s): 1-6

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Title

A method of GC-MS analysis of serum metabolites

Author(s)

Keywords

metabolomics

rat

serum

GC-MS

Abstract

Introduction: Metabolites are the intermediate and natural low molecular weight products of metabolic reactions which naturally occur within cells. metabolomics is a post-genomics study that analyzes metabolic profile in all biological samples. Gas chromatography-mass spectrometry (GC-MS) is a powerful technique that detects volatile components. This study evaluates the metabolite profile of serum with a simple method for derivatization. Methods: In this study, the metabolic profile of serum sample of 16 rats was analyzed using GC-MS after protein precipitation, using acetonitrile, and derivatize with a method of less in number and volume of materials. Acquisition chromatograms were pre-processed and analyzed using National Institute of Standards and Technology (NIST) library. Results: 35 metabolites with probability of >60% perfect match were detected according to spectrum fragments analysis by NIST library. Conclusion: This study represents the metabolome profile of serum instead of the change of metabolites that can be evaluated with a simple derivatization method.

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APA: Copy

SOHEILI, MASOUD, AREFI OSKOUIE, AFSANEH, REZAEI TAVIRANI, MOSTAFA, ALIAKBARZADEH, GHAZALEH, & SALAMI, MAHMOUD. (2018). A method of GC-MS analysis of serum metabolites. ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES), 9(1), 1-6. SID. https://sid.ir/paper/315918/en

Vancouver: Copy

SOHEILI MASOUD, AREFI OSKOUIE AFSANEH, REZAEI TAVIRANI MOSTAFA, ALIAKBARZADEH GHAZALEH, SALAMI MAHMOUD. A method of GC-MS analysis of serum metabolites. ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES)[Internet]. 2018;9(1):1-6. Available from: https://sid.ir/paper/315918/en

IEEE: Copy

MASOUD SOHEILI, AFSANEH AREFI OSKOUIE, MOSTAFA REZAEI TAVIRANI, GHAZALEH ALIAKBARZADEH, and MAHMOUD SALAMI, “A method of GC-MS analysis of serum metabolites,” ARCHIVES OF ADVANCES IN BIOSCIENCES (JOURNAL OF PARAMEDICAL SCIENCES), vol. 9, no. 1, pp. 1–6, 2018, [Online]. Available: https://sid.ir/paper/315918/en

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