مرکز اطلاعات علمی Scientific Information Database (SID) - Trusted Source for Research and Academic Resources

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Information Journal Paper

Title

DETECTION OF STAPHYLOCOCCUS ENTEROTOXIN B (SEB) USING AN IMMUNOCHROMATOGRAPHIC TEST STRIP

Pages

  1-6

Abstract

 Background: STAPHYLOCOCCUS AUREUS is one of the most important microorganisms that causes various human diseases by secreting virulence factors known as staphylococcal super antigens (SAgs). STAPHYLOCOCCAL ENTEROTOXIN B (SEB) is a bacterial antigen that is responsible for food poisoning in humans. Among SEB detection methods, a lateral flow device (LFD) is ideal for rapid immunochromatographic tests because it is easy to use, requires minimal time to produce results, and does not require personnel training.Objectives: In our laboratory, the production of an immunochromatographic test strip, for the detection of SEB using a sandwich assay and a competitive method, was described; the test can detect SEB with high sensitivity.Materials and Methods: The strip assays were compared with PCR, a valid method for detection. For PCR, a specific sequence for SEB production was detected using primers designed according to GenBank sequences.Results: In total, 80 food samples suspected of SEB contamination were assessed using the two methods. Fifty-four samples were contaminated based on the PCR technique and twenty-six of those were confirmed using the strip assay.Conclusions: The sensitivity of the sandwich method was approximately 10 ng/mL and that of the competitive method was approximately 250 ng/mL. In the LFD, a highly specific monoclonal antibody used for both the sandwich and competitive methods resulted in an increased sensitivity and accuracy for the detection of a minimal SEB concentration.

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    Cite

    APA: Copy

    GHOLAMZAD, MEHRDAD, KHATAMI, MOHAMMAD REZA, GHASSEMI, SOHEIL, VAISE MALEKSHAHI, ZIBA, & BARAT SHOOSHTARI, MOHAMMAD. (2015). DETECTION OF STAPHYLOCOCCUS ENTEROTOXIN B (SEB) USING AN IMMUNOCHROMATOGRAPHIC TEST STRIP. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(9), 1-6. SID. https://sid.ir/paper/317487/en

    Vancouver: Copy

    GHOLAMZAD MEHRDAD, KHATAMI MOHAMMAD REZA, GHASSEMI SOHEIL, VAISE MALEKSHAHI ZIBA, BARAT SHOOSHTARI MOHAMMAD. DETECTION OF STAPHYLOCOCCUS ENTEROTOXIN B (SEB) USING AN IMMUNOCHROMATOGRAPHIC TEST STRIP. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(9):1-6. Available from: https://sid.ir/paper/317487/en

    IEEE: Copy

    MEHRDAD GHOLAMZAD, MOHAMMAD REZA KHATAMI, SOHEIL GHASSEMI, ZIBA VAISE MALEKSHAHI, and MOHAMMAD BARAT SHOOSHTARI, “DETECTION OF STAPHYLOCOCCUS ENTEROTOXIN B (SEB) USING AN IMMUNOCHROMATOGRAPHIC TEST STRIP,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 9, pp. 1–6, 2015, [Online]. Available: https://sid.ir/paper/317487/en

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