CONSTRUCTION OF A DNA VACCINE ENCODING MTB32C AND HBHA GENES OF MYCOBACTERIUM TUBERCULOSIS

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TEIMOURPOUR ROGHAYEH; SADEGHIAN ALI; MESHKAT ZAHRA; ESMAELIZAD MAJID; SANKIAN MOJTABA; JABBARI AHMAD REZA

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Journal: Jundishapur Journal of Microbiology Year:2015 | Volume:8 | Issue:8 Page(s): 1-7

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Title

CONSTRUCTION OF A DNA VACCINE ENCODING MTB32C AND HBHA GENES OF MYCOBACTERIUM TUBERCULOSIS

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Keywords

VACCINES

DNA

HBHA PROTEIN

MTB32 ANTIGEN

MYCOBACTERIUM TUBERCULOSIS

Abstract

Background: Tuberculosis (TB) is a contagious disease caused by MYCOBACTERIUM TUBERCULOSIS. Development of a new vaccine for tuberculosis requires immunogenic antigens capable of inducing suitable and long-lasting T cell immunity. The emergence of multidrugs and extensively drug-resistant strains of M. tuberculosis has made it a global public health concern.Objectives: DNA vaccine is a straightforward method to introduce antigens to the host. In the present study, two immunodominant mycobacterial antigens (Mtb32C and HBHA) were isolated and cloned into pcDNA3.1 (+) to design and construct a new DNA vaccine. This vector is capable of producing new potent chimeric protein.Materials and Methods: Mtb32C (Rv0125) and heparin-binding haemagglutinin adhesion (HBHA) genes were amplified using polymerase chain reaction (PCR) of M. tuberculosis H37Rv genome and ligated into pcDNA3.1 (+). Colony-PCR and restriction enzyme analysis were used to confirm the accuracy of the cloning procedure.Results: In the current study, recombinant pcDNA3.1 (+) vector containing Mtb32C and HBHA genes was successfully constructed. The desired size of DNA fragment was observed using single and double digestion methods.Conclusions: Mtb32C and HBHA genes were successfully isolated from H37Rv genome and cloned into an eukaryotic expression vector. This vector can be considered as a vaccine to evaluate immune responses in animal models.

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APA: Copy

TEIMOURPOUR, ROGHAYEH, SADEGHIAN, ALI, MESHKAT, ZAHRA, ESMAELIZAD, MAJID, SANKIAN, MOJTABA, & JABBARI, AHMAD REZA. (2015). CONSTRUCTION OF A DNA VACCINE ENCODING MTB32C AND HBHA GENES OF MYCOBACTERIUM TUBERCULOSIS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 8(8), 1-7. SID. https://sid.ir/paper/317503/en

Vancouver: Copy

TEIMOURPOUR ROGHAYEH, SADEGHIAN ALI, MESHKAT ZAHRA, ESMAELIZAD MAJID, SANKIAN MOJTABA, JABBARI AHMAD REZA. CONSTRUCTION OF A DNA VACCINE ENCODING MTB32C AND HBHA GENES OF MYCOBACTERIUM TUBERCULOSIS. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2015;8(8):1-7. Available from: https://sid.ir/paper/317503/en

IEEE: Copy

ROGHAYEH TEIMOURPOUR, ALI SADEGHIAN, ZAHRA MESHKAT, MAJID ESMAELIZAD, MOJTABA SANKIAN, and AHMAD REZA JABBARI, “CONSTRUCTION OF A DNA VACCINE ENCODING MTB32C AND HBHA GENES OF MYCOBACTERIUM TUBERCULOSIS,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 8, no. 8, pp. 1–7, 2015, [Online]. Available: https://sid.ir/paper/317503/en

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