STAPHYLOCOCCUS AUREUS ENTEROTOXIN B DOWN-REGULATES THE EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA (TGF-β) SIGNALING TRANSDUCERS IN HUMAN GLIOBLASTOMA

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AKBARI ABOLFAZL; FARAHNEJAD ZOHREH; AKHTARI JAVAD; ABASTABAR MAHDI; MOBINI GHOLAMREZA; ALI MEHBOD AMIR SAYED

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Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:5 Page(s): 0-0

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Title

STAPHYLOCOCCUS AUREUS ENTEROTOXIN B DOWN-REGULATES THE EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA (TGF-β) SIGNALING TRANSDUCERS IN HUMAN GLIOBLASTOMA

Author(s)

Keywords

CELL SIGNALING TRANSDUCERS

GLIOBLASTOMA

TRANSFORMING GROWTH FACTOR-β (TGF-β)

STAPHYLOCOCCUS AUREUS ENTEROTOXIN B

Abstract

Background: It has been revealed that STAPHYLOCOCCUS AUREUS ENTEROTOXIN B (SEB) may feature anti-cancer and anti-metastatic advantages due to its ability to modify cell immunity processes and signaling pathways. GLIOBLASTOMA is one of the most aggressive human cancers; it has a high mortality nature, which makes it an attractive area for the development of novel therapies.Objectives: We examined whether the SEB could exert its growth inhibitory effects on GLIOBLASTOMA cells partially through the manipulation of a key tumor growth factor termed transforming growth factor-beta (TGF-b).Materials and Methods: A human primary GLIOBLASTOMA cell line, U87, was treated with different concentrations of SEB. The cell quantity was measured by the MTT assay at different exposure times. For molecular assessments, total ribonucleic acid (RNA) was extracted from either non-treated or SEB-treated cells. Subsequently, the gene expression of TGF-b transducers, smad2/3, at the messenger RNA (mRNA) level, was analyzed via a quantitative real-time polymerase chain reaction (qPCR) using the SYBR Green method. Significant differences between cell viability and gene expression levels were determined (Prism 5.0 software) using one-way analyses of variance (ANOVA) test.Results: We reported that SEB could effectively down-regulate smad2/3 expression in GLIOBLASTOMA cells at concentrations as quantity as 1mg/mL and 2mg/mL (P<0.05 and P<0.01, respectively). The SEB concentrations effective at regulating smad2/3 expression were correlated with those used to inhibit the proliferation of GLIOBLASTOMA cells. Our results also showed that SEB was able to decrease smad2/3 expression at the mRNA level in a concentration- and time-dependent manner.Conclusions: We suggested that SEB could represent an agent that can significantly decrease smad2/3 expression in GLIOBLASTOMA cells, leading to moderate TGF-b growth signaling and the reduction of tumor cell proliferation.

Cites

THE ROLE OF MICRORNA SIGNATURE AS DIAGNOSTIC BIOMARKERS IN DIFFERENT CLINICAL STAGES OF COLORECTAL CANCER

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APA: Copy

AKBARI, ABOLFAZL, FARAHNEJAD, ZOHREH, AKHTARI, JAVAD, ABASTABAR, MAHDI, MOBINI, GHOLAMREZA, & ALI MEHBOD, AMIR SAYED. (2016). STAPHYLOCOCCUS AUREUS ENTEROTOXIN B DOWN-REGULATES THE EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA (TGF-β) SIGNALING TRANSDUCERS IN HUMAN GLIOBLASTOMA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(5), 0-0. SID. https://sid.ir/paper/317617/en

Vancouver: Copy

AKBARI ABOLFAZL, FARAHNEJAD ZOHREH, AKHTARI JAVAD, ABASTABAR MAHDI, MOBINI GHOLAMREZA, ALI MEHBOD AMIR SAYED. STAPHYLOCOCCUS AUREUS ENTEROTOXIN B DOWN-REGULATES THE EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA (TGF-β) SIGNALING TRANSDUCERS IN HUMAN GLIOBLASTOMA. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(5):0-0. Available from: https://sid.ir/paper/317617/en

IEEE: Copy

ABOLFAZL AKBARI, ZOHREH FARAHNEJAD, JAVAD AKHTARI, MAHDI ABASTABAR, GHOLAMREZA MOBINI, and AMIR SAYED ALI MEHBOD, “STAPHYLOCOCCUS AUREUS ENTEROTOXIN B DOWN-REGULATES THE EXPRESSION OF TRANSFORMING GROWTH FACTOR-BETA (TGF-β) SIGNALING TRANSDUCERS IN HUMAN GLIOBLASTOMA,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 5, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317617/en

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