Study of Genetic Variation of Leishmania major Based on Internal Transcribed Spacer 1 (ITS1) in Chabahar, Iran

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DABIRZADEH MANSOUR; HASHEMI MOHAMMAD; MAROUFI YAHYA

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Journal Paper

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Journal: Jundishapur Journal of Microbiology Year:2016 | Volume:9 | Issue:6 Page(s): 0-0

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Title

Study of Genetic Variation of Leishmania major Based on Internal Transcribed Spacer 1 (ITS1) in Chabahar, Iran

Author(s)

DABIRZADEH MANSOUR | HASHEMI MOHAMMAD | MAROUFI YAHYA | Issue Writer Certificate

Keywords

Cutaneous Leishmaniasis

ITS1

PCR-RFLP

Genetic Variation

Abstract

Objectives: This study investigates the determination of Genetic Variation within the species of Leishmania major isolates from new cases in Chabahar, a port city in Southeast Iran (situated at the Iran-Pakistan border). Migration in this region indicates that leishmaniasis is spreading gradually, and a new micro-habitat focus appears each year. Materials and Methods: A variety of nucleic acid detection methods that target bothDNAand RNA have been developed. The restriction fragment length polymorphism analysis of amplified internal transcribed spacer 1 with polymerase chain reaction (ITS1-RFLP PCR) assay is a multipurpose tool for the diagnosis of Leishmania from clinical samples and for enabling the determination of the infecting Leishmania species. The goal of this study was the identification of species based on ITS1-RFLP in the ribosomal operon of L. major from clinically different forms of ZCL amplified by PCR, followed by the digestion of the PCR product with restriction enzymes. The profiles were observed and visualized in agarose gel under UV light. We used direct smears to identify the parasites. While taking the smear, samples were collected for culture or direct PCR. We used the PCR-RFLP assay of the ITS1 genes for direct identification of Leishmania species in 24 out of 33 suspected patients. PCR-ITS1 amplification was done on the 24 samples confirmed by culture via growth and parasitological methods. Results: Of the 24 isolates, 21 had 350 bp bands (87. 5%) and three had 450 bp bands (12. 5%). After using the restriction enzyme, banding patterns including fragments of 210 and 140 bp for L. major were detected in 19 cases. Conclusions: The L. major species causing ZCL in Chabahar have limited Genetic Variation. There seems to be little manifestation of diversity between these lesions as a new focus of disease, and new micro-habitats for the disease are appearing in parts of this region.

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APA: Copy

DABIRZADEH, MANSOUR, HASHEMI, MOHAMMAD, & MAROUFI, YAHYA. (2016). Study of Genetic Variation of Leishmania major Based on Internal Transcribed Spacer 1 (ITS1) in Chabahar, Iran. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), 9(6), 0-0. SID. https://sid.ir/paper/317888/en

Vancouver: Copy

DABIRZADEH MANSOUR, HASHEMI MOHAMMAD, MAROUFI YAHYA. Study of Genetic Variation of Leishmania major Based on Internal Transcribed Spacer 1 (ITS1) in Chabahar, Iran. JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM)[Internet]. 2016;9(6):0-0. Available from: https://sid.ir/paper/317888/en

IEEE: Copy

MANSOUR DABIRZADEH, MOHAMMAD HASHEMI, and YAHYA MAROUFI, “Study of Genetic Variation of Leishmania major Based on Internal Transcribed Spacer 1 (ITS1) in Chabahar, Iran,” JUNDISHAPUR JOURNAL OF MICROBIOLOGY (JJM), vol. 9, no. 6, pp. 0–0, 2016, [Online]. Available: https://sid.ir/paper/317888/en

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